FastQCFastQC Report
Sat 30 Sept 2023
SRR9257377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9257377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20227716
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length55
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG6974203.4478435429882444TruSeq Adapter, Index 12 (100% over 49bp)
TGGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTC6315893.122394045872505Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCT3649771.8043411327309518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGT3618091.7886794534785835No Hit
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTC3121671.5432637080726266No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTC2850691.4092990034070085No Hit
TGATCCGGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCT2554561.262900863350069Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTCC2553831.2625399723824478Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGTTCGATCCTTCCCAGTAAAACGCGGAGATCGGAAGAGCACACGTCTG2460261.2162816602724695Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGT2425421.1990577680643726No Hit
GATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTCT2373501.1733900159563244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGTC2290871.132540124648774No Hit
AGGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT2219161.0970887667198808Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTCC2166021.0708178817618361Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGATGAGTGTTCGCCCTTGGTCTAAGATCGGAAGAGCACACGTCTGAACT2090781.0336213935374612Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGTC1496560.7398561458940792No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGT1406920.6955407125549914No Hit
TGGGTTGGGCCAACCTATGGTGTACAGATCGGAAGAGCACACGTCTGAAC1367940.6762701236264144Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGTCT1343470.6641728606432876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACG1171800.5793041587097624No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGTC1092480.5400906360362189No Hit
GAGGTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGT979760.48436511566604945No Hit
GATCCGGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCTG821070.4059133517595363Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTGAGATCGGAAGAGCACACGT813010.4019287199800511No Hit
GAGCGTAATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTC788610.38986606298012094No Hit
AGCGTAATGGTATAAGCCTGCCTGACTGTAGATCGGAAGAGCACACGTCT752930.3722268989736656Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAAGATCGGAAGAGCACACGTCT735680.3636989959716658Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGTCTG699620.34587197091357225Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAG693800.3429947305963758Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACAC677210.3347931125788003No Hit
CTGGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGT624970.30896716169042515No Hit
AGGTGTGAAGCCATCTCGTACTAAACAGATCGGAAGAGCACACGTCTGAA619380.3062036267465887Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACACG591050.2921980909757681No Hit
GAATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGT577300.28540048713359434No Hit
GCGGAAATAGCTTAATGGTAGAGCATAGCCAGATCGGAAGAGCACACGTC575360.2844414070278622No Hit
AAGAAGGTTGAAACTTCCAGGAGATCGGAAGAGCACACGTCTGAACTCCA568090.2808473284873092Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGATGAGTGTTCGCCCTTGGTCTAGATCGGAAGAGCACACGTCTGAACTC565960.2797943178557579Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGGGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACT559960.2768280907246275Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGA558210.2759629411447145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGTCT534040.2640139895181443Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTG521460.257794799966541Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTGTAGCGCAGTCTGGTCAGATCGGAAGAGCACACGTCTGAACTCCA516870.2555256362112262Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTGAAGATTACAGGTTCGAATCCTGTCCCCGCAGATCGGAAGAGCACAC508780.25152617329608545No Hit
ACGATGAGTGTTCGCCCTTGGTCTAAGATCGGAAGAGCACACGTCTGAAC505730.2500183411710941Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATGGCCCGAGGTAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT491920.24319107505760906Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGAAGCCATCTCGTACTAAACGAAAAGATCGGAAGAGCACACGTCTGAA482770.23866757868263525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCTAACGGCGTACCTTTTAGATCGGAAGAGCACACGTCTGAACTCCAG471840.23326410159209277Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACG471360.2330268034216023No Hit
AGCGCCGGGCAGCTAAGTTGGTATGAGATCGGAAGAGCACACGTCTGAAC471350.2330218597097171Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACG458090.22646649774991898No Hit
ATCCGGGAGTCCCGTGTGGAAGGGCTCTCGAGATCGGAAGAGCACACGTC448010.22148323616961993No Hit
AACGATGAGTGTTCGCCCTTGGTCTAAGATCGGAAGAGCACACGTCTGAA445470.22022753335077475Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTTCGATCCTTCCCAGTAAAACGCGGCAGATCGGAAGAGCACACGTCT433960.21453732097088965Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGATGATGGAAAAACCAGAGATCGGAAGAGCACACGTCTGAACTCCAG414400.20486742052340462Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCGAGAGGTACGGGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT409320.20235601488571425Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGGTTGGGCCAACCTATGGTGTACCAGATCGGAAGAGCACACGTCTGAA407240.20132772281358904Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTCCA400850.19816869091893516Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTCTAACGGCGTACCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGT398990.19724916050828478Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTCCGATGGGTACACGAAAGTGACGAAGTAGATCGGAAGAGCACACGTC389750.192681170726344No Hit
AGGGAGAACTGCGAGGAGCCAACCCTAGATCGGAAGAGCACACGTCTGAA386240.1909459278546327Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGCATTGGATGGATGCCCGGGCATTGAGAGATCGGAAGAGCACACGTC385500.19058009317512664No Hit
GGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCTGAACTC384750.19020931478373534Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGTTGGGCCAACCTATGGTGTACAGATCGGAAGAGCACACGTCTGAACT382270.18898327423620145Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCCGTTCTCGTTTGTACAGACAGACTTAGATCGGAAGAGCACACGTCT369300.18257127992107464Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTCTGGGTCCCTACCGACGCTGGGGTGCAGATCGGAAGAGCACACG367700.18178028601943985No Hit
CTGGGTTGGGCCAACCTATGGTGTACCGGTTGAGATCGGAAGAGCACACG363220.17956550309486252No Hit
GCGGAAATAGCTTAATGGTAGAGCATAGCAGATCGGAAGAGCACACGTCT361450.17869046609117906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTGTGAAGCCATCTCGTACTAGATCGGAAGAGCACACGTCTGAACTC357210.17659433225184692Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACACGT353680.17484920195636522No Hit
GTGATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGT351540.17379124761292872No Hit
AGGTGTGAAGCCATCTCGTACTAAACGAACAGATCGGAAGAGCACACGTC348970.17252071365842786No Hit
AGGTGTGAAGCCATCTCGTACAGATCGGAAGAGCACACGTCTGAACTCCA343020.16957920508672358Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAAACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTC342620.16938145661131487No Hit
AGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACGTCTGAACTCCAGT339940.16805654182607666Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGT331860.16406202262282107No Hit
CGAAGACACGGGTTCGATTCCCGTAAGGGAGATCGGAAGAGCACACGTCT330790.16353304545110284Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGATCGGATATAGCTGGTTTTCCGCGAAATCAGATCGGAAGAGCACACG322090.1592320161109638No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAATCAGATCGGAAGAGCACACGT320560.15847562819252553No Hit
TTGGATGATGGAAAAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC303900.15023940419175355Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGTGTAGCGCAGTCTGGTCAGCGCATCTGAGATCGGAAGAGCACACGTC301660.14913201272946486No Hit
ATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCTGAACTCCAGT296070.1463684777856284Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAAGTGTTCACTTCTTTTTCGCCAGGTTTCATAGATCGGAAGAGCACAC291560.1441388637253954No Hit
ATCCGGGAGTCCCGTGTGGAAGGGCTCTAGATCGGAAGAGCACACGTCTG289790.14326382672171192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTCTCTGGGTCCCTACCGACGCTGAGATCGGAAGAGCACACGTCTGAA284030.14041624867582675Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAAGATCGGATATAGCTGGTTTTCCGCGAAAAGATCGGAAGAGCACACGT283390.14009985111517287No Hit
TGGGTTGGGCCAACCTATGGTGTACCGGTAGATCGGAAGAGCACACGTCT282440.13963019848607724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAAC281610.13921987039960418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCGAAGCGACGAGACCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG280220.13853269444755897Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGCCGTTCTCGTTTGTACAGACAGACTTTAGATCGGAAGAGCACACGTC279480.1381668597680529No Hit
TGCGAGAGGCACGGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT273990.1354527619430686Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCAGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCT273970.13544287451929818Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGATCCGGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTC268000.13249147852382345No Hit
ACCTGAAGATTACAGGTTCGAATCCTGTCCCCGCAGATCGGAAGAGCACA267480.13223440550579216No Hit
AGTTCTTCCTATTTGACCCGAAACCGATCGATAGATCGGAAGAGCACACG265980.13149284872300956No Hit
GAGGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTC263740.13038545726072087No Hit
AGTAAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCTGAAC263350.13019265249719741Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAAGATCGGATATAGCTGGTTTTCCGCGAAATAGATCGGAAGAGCACACG262590.1298169303939209No Hit
CGATGAGTGTTCGCCCTTGGTAGATCGGAAGAGCACACGTCTGAACTCCA258090.1275922600455731Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTTGGGCCAACCTATGGTGTAGATCGGAAGAGCACACGTCTGAACTCCA257040.1270731702976253Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTGTGAAGCCATCTCGTACTAAACGAAAAAGATCGGAAGAGCACACGT248520.12286112777142016No Hit
GGTGTGAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTG246930.1220750775816706Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGCGGCGTGTTCGAATTCAGATCGGAAGAGCACACGTCTGAACTCC245860.12154610040995235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCT243260.12026073531979586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTCT240830.11905941333168807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCATTGGATGGATGCCCGGGCATTGAGAAGATCGGAAGAGCACACGTCT239440.11837223737964286Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATCCGGGAGTCCCGTGTGGAAGGGCTCTCGCAGATCGGAAGAGCACACG239310.11830796912513503No Hit
CGGGAGTCCCGTGTGGAAGGGCTCAGATCGGAAGAGCACACGTCTGAACT235270.11631070952350725Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAACGCGGCGTGTTCGAATTCTGATAGATCGGAAGAGCACACGTCTGAA233610.11549005335056119Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGAGTGTTCGCCCTTGGTCTAGATCGGAAGAGCACACGTCTGAACT227050.11224697835385863Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGTGTCAGTAGCGGAACGTTCTGTCAAGATCGGAAGAGCACACGTCTGA224860.11116430545099604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGCGGCGTGTTCGAATTCTGATCGCTAGATCGGAAGAGCACACGTCTG224530.11100116295878389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAGT222360.10992837747969174Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGCGCTTGAGAGAACCATGTCGAAGGAACAGATCGGAAGAGCACACGTC221670.10958726135961173No Hit
AGATCGGATATAGCTGGTTTTCCGCGAAAGATCGGAAGAGCACACGTCTG214170.10587947744569876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAGCCATCTCGTACTAAACGAAAGATCGGAAGAGCACACGTCTGAACTC213690.10564217927520833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGAAGATTACAGGTTCGAATCCTGTCCCCGCAGATCGGAAGAGCACACG213610.10560262958012659No Hit
AGTAGAGCGTATGATGTCGATGGCCAGATCGGAAGAGCACACGTCTGAAC213180.10539004996906225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGTTGGGCCAACCTATGGTGTACCGGTTGTAGATCGGAAGAGCACACG206980.10232494860022753No Hit
AGGTGTAGCGCAGTCTGGTCAGATCGGAAGAGCACACGTCTGAACTCCAG206590.10213214383670405Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGTTGGGCCAACCTATGGTGTACCGGTTAGATCGGAAGAGCACACGTCT205810.1017465343096571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGTGAAGCCATCTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC205470.10157844810555972Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGAAGCCATCTCGTACTAAACGAAACAGATCGGAAGAGCACACGTCTGA204550.10112362661211974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)