FastQCFastQC Report
Thu 28 Sept 2023
SRR9295891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36096491
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA18385975.093561587468433No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA15583364.317139857167834No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT6393331.771177702563942No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG4785881.3258574081342145No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG4578151.2683088780014655No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA4318231.1963018787615671No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGAT3125630.8659096531017377No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG2956890.8191627269254509No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2758520.7642072466268259No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA2584360.7159587894568478No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG2526910.7000431149941971No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC2083630.5772389343883869No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA2080060.5762499185862693No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT2077190.5754548274512333No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT2011250.5571871238121179No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1943370.5383819718099468No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1943170.5383265647622091No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1808820.5011068804444178No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT1723050.47734556802211053No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA1679070.4651615582245931No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1653210.45799742695211015No Hit
ACGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1648050.4565679251204778No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1576750.43681531260199224No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1539830.4265871715896151No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA1519840.4210492371682333No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA1486010.41167713504340353No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA1473770.4082862237218571No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC1467360.4065104278418642No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1390690.38527013609162175No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1301580.3605835259720952No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1247320.3455515939208606No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1238630.3431441576966581No Hit
AAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA1196940.33159455859573717No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1189230.32945861690544936No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAG1145470.31733555486044335No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT1145290.31728568851747946No Hit
TTAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT1131290.3134071951758413No Hit
TAAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT1127140.3122574989352843No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA1025180.28401098599861135No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1019710.28249560324298556No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA1017150.2817863930319432No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA992160.27486328241711916No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG959160.2657211195404008No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC932410.25831042690548506No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA927390.2569197100072691No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT897870.2487416297611865No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAAT897640.24867791165628814No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC887660.24591309997417754No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT872630.24174926033669036No Hit
ACCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG871500.24143621051697242No Hit
TAGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA821150.22748748624900963No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA818200.22667023229487873No Hit
TACGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC804340.222830523886657No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG802190.22223489812347685No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG783830.21714853114115718No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGA783620.21709035374103258No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT765490.21206770486361126No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC756410.20955222489632028No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC746300.20675139863318018No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATC734320.20343251647369268No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATA709180.1964678505730654No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA687180.1903730753219198No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAAT670720.18581307529310814No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATAGATCGGAAGAGC640800.17752418095155012No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC632020.17509181155586565No Hit
TATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAA624590.17303343973241056No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT621770.17225220035930916No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA618570.1713656875955062No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA609140.1687532452946742No Hit
ACCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA606470.1680135612073761No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA605420.16772267420675321No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT604930.1675869269397959No Hit
TACGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGA604620.16750104601580248No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATA603750.16726002535814355No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA600500.1663596608324061Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA578440.1602482634669392No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG577510.15999062069495898No Hit
TATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCA573670.15892680537839537No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT567370.1571814833746582No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATAG564300.15633098519188474No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATAG549470.1522225526021352No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA549110.15212281991620738No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT536010.14849365828938885No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA527090.14602250396028799No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT512290.14192238242769914No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA511670.1417506205797123No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAA497790.1379053714667168No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG488400.1353040105754324No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT484410.13419863997306553No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA477620.13231757070237105No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA476910.13212087568290226No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATA473910.13128976996683694No Hit
ACGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA473770.13125098503342056No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA467420.129491811267749No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTAGGCACCATCAAT461460.1278406812451659No Hit
ATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA456320.12641672011830735No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT455040.12606211501278614No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGCTGTAGGCACCATCAATAG443010.12272938109136426No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT439100.12164617330809246No Hit
TACGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAG435230.12057404693436823No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT430820.11935232153175222No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT429730.11905035312158181No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA423270.11726070547965452No Hit
TATGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCAT417200.1155791015808157No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA417030.11553200559023867No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC412460.11426595454943252No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC411910.11411358516815388No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT411520.11400554142506539No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC407540.1129029411750854No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC402200.11142357300048918No Hit
GGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCG397860.11022124006458134No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAAT395720.1096283846537881No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCA390700.10823766775557216No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG382930.106085103950963No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT378900.10496865193904859No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA378850.10495480017711416No Hit
TGGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA378580.10488000066266828No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT374950.10387436274622926No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT372370.10315961183041311No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATA371930.10303771632539017No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATAG367600.10183815374186925No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)