FastQCFastQC Report
Thu 28 Sept 2023
SRR9295892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31850789
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA13595634.268537900269911No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA12288653.8581932774098626No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT6802022.135589168607409No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG6381242.0034794114519423No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG3286321.0317860571680029No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT3148400.9884841471274072No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3134140.9840070209877689No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA2875930.9029383856079672No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2834130.889814691874666No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA2676180.8402240836168926No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG2482520.779421822172129No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC2384140.7485340473041343No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGAT2347740.7371057589813552No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATA2247370.7055931958231867No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAATA2156290.6769973578990461No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG2147150.6741277272597548No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC2016310.6330486820907325No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1923460.6038971279486985No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA1884300.5916022990827637No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1831910.5751537269610496No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1803100.5661084251319489No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1776250.5576784926740747No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1758410.5520773755400533No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA1725430.5417228439772717No Hit
TCGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1635510.5134912042524283No Hit
TGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT1598280.5018023258387728No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1570980.4932311095966885No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT1518990.4769081230609389No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC1418370.4453170689115425No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA1280820.4021313255379639No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC1256840.39460246965938583No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1232090.3868318615278259No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1179300.3702577038201471No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCG1146730.36003189748297915No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1121820.3522110551170333No Hit
GAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA1088570.34177175328372555No Hit
ACCCCTCCCACGGCGCGACCCTGTAGGCACCATCAATAGATCGGAAGAGC1048230.3291064469391951No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA1040160.3265727577423592No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1016530.3191537892515002No Hit
ACGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA996740.31294044238590135No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC986140.3096124243578393No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA960040.3014179648736488No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT920140.28889080267367945No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGA911460.2861655954582475No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGAT903990.2838202846403585No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC899810.2825079152670284No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA894290.28077483418071686No Hit
TTAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT887840.2787497666070376No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA864440.27140300982810817No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAG862780.2708818296463551No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG857630.26926491522706075No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAAT828270.2600469332172588No Hit
AAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATA743000.23327522592925407No Hit
TAAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAAT673720.21152380244018446No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA665680.20899953216229591No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC648770.20369040151564222No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT623350.19570943752759154No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATC622900.19556815374338135No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT615250.19316632941180828No Hit
ACCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG612180.19220246003952995No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT606510.19042228435848166No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA604600.18982261318550067No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT602080.18903142399392367No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATA595100.1868399555188413No Hit
GGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGG592980.1861743519132289No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGA574380.18033462216587476No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA571610.1794649419830699No Hit
TAGCCTCGGTTGGCCTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGA567290.17810861765465213No Hit
CAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTATCTCG563070.17678368972272554TruSeq Adapter, Index 2 (97% over 45bp)
CGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC547990.17204911313185994No Hit
TCGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATA544450.17093768069607318No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA526500.16530202752591153No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAAT521240.16365057707047698No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA519480.16309800049223272Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATC516520.16216866715609463No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT508080.15951881129224146No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT506770.15910751849820737No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAG506650.15906984282241798No Hit
TACGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC504530.1584042392168056No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT497210.1561060229936533No Hit
ACGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGAT494690.15531483380207628No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT491600.15434468515049973No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCTGTAGGCACCATCAATAGATCGGA480290.15079375270735051No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT470730.14779225720279646No Hit
ACCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA466590.1464924463880628No Hit
TCGGGGGGGATGCGTGCATTTATCAGATCCTGTAGGCACCATCAATAGAT455480.14300430673789588No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC455320.14295407250351003No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG432220.13570150491405408No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT431450.1354597526610722No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAA428630.13457437428002175No Hit
GGGGCCGCCGGTGAAATACCACTACTCTGATCTGTAGGCACCATCAATAG425390.13355713103370845No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA425100.1334660814838841No Hit
TCGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCA422610.13268431121125446No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA417790.13117100490038097No Hit
TGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA415820.13055249588950527No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT414640.13018201841090968No Hit
TAGAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAA412650.1295572301207358No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT411540.129208730119684No Hit
CAGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAA407010.12778647335863486No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATA405950.12745367155582865No Hit
TCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGA399840.12553535173021932No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAAT397610.12483521208846662No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATA391070.12278188775794534No Hit
TACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAAT382790.12018226612847802No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC381270.11970504090181253No Hit
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC376250.11812894179795673No Hit
ACCCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC360330.11313063547656542No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTAGGCACCATCAAT359910.11299877061130259No Hit
ACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAAT359270.11279783367375922No Hit
ACGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA359100.11274445979972426No Hit
TACGGGCCGCCGGTGAAATACCACTACTCTCTGTAGGCACCATCAATAGA357550.11225781565411141No Hit
AATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACGTATCTCGT349420.10970528861938082TruSeq Adapter, Index 2 (97% over 46bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGA338330.10622342824851215No Hit
GCGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC338030.1061292390590387No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT335730.10540712193974221No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATAG333800.104801171487463No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGCTGTAGGCACCATCAATAGAT333670.1047603561720245No Hit
TATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAA329540.10346368499693996No Hit
TACCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCTGTAGGCACCATCAA328470.10312774355448462No Hit
ACCCCTCCCACGGCGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA324290.10181537418115451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)