FastQCFastQC Report
Thu 28 Sept 2023
SRR9295897.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295897.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23849660
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA6044112.5342541570823234No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4787572.0073954932690863Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT2329380.9766931687915047No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC1956700.820430983083197No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1852380.776690317597819No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1776470.7448617716143542No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT1651260.6923620714089844No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1511870.6339167937823851No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1460470.612365123863401No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1434810.6016060606314724No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA1361890.5710312012833726No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG1064080.4461614966418809No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG1059940.4444256228390678No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1058790.44394343567161965No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC1023250.4290417557315283No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA941720.3948567820254041No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG891120.3736405466576882No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC870180.36486054727824213No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA845800.35463817932834263No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC781120.3275182958583057No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA768190.3220968349234329No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG690520.289530332927178No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT678430.2844610782711368No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA633630.2656767434001156No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG626880.2628465143737898No Hit
TCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA558640.23423394715060925No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC547130.22940788254423752No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATA527280.2210849127408944No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT520360.2181834038724242No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC514000.2155166991898417No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATA490940.2058477982495348No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC461730.19360024419635333No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA450020.1886903209521645No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT448930.18823329137606154No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA446260.18711377856120381No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA444460.18635905082085027No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT443510.18596072229121924No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT442300.18545337753242602No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACC440080.1845225466526567No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACC408440.17125611014999795No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC385560.1616626819837264No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA384310.16113856549736977No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT383340.16073185110395705No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA380970.1597381262458249Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAAT378240.15859345583962203No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT364630.15288687553617117No Hit
CCCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC345350.14480290285060668No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT342840.1437504769460026No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA339550.14237100235391195No Hit
TCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATA338260.14183011413999194No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCA337230.14139824215523408No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA336190.14096217723858537No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA330800.13870218694941563No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA330800.13870218694941563No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA327780.13743592151837805No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT327710.13740657099514206No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGA326950.13708790817143723No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA318890.13370840506740977No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAAT312450.13100815692970044No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG307460.12891588391616485No Hit
GCTCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA301050.1262282145741281No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAGA295070.12372084130339803No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA291180.12208979079785624No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA290460.12178789970171483No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA283060.11868513010248365No Hit
TCACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGA269620.11304982964117727No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC266360.11168293384475922No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA262200.10993867417816439No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA260400.10918394643781085No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT258240.10827827314938661No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA258140.1082363438304781No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA257490.10796380325757264No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGA255860.10728035535936362No Hit
TGTTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT255300.10704555117347583No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC252440.10584637265269191No Hit
GGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG242520.10168698421696577No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)