FastQCFastQC Report
Thu 28 Sept 2023
SRR9295898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36991236
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA7890232.132999827310447No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT5848351.5810096207653077No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT5668491.5323872930334093No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4874881.3178472868546485Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA4577611.2374850086112288No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3236540.8749477849293816No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT2792930.7550247847895647No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG2630620.7111468240747619No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA2579350.6972867843615714No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT2503120.6766792004462895No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC2432740.6576530722033727No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT2083180.5631550132577349No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC2022650.5467916778990569No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1968580.532174702137555No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1878080.5077094477189137No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC1732360.4683163330903569No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG1702850.4603387678097591No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1623630.4389228843286015No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1619860.43790372400641064No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA1556320.42072668239579775No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1544830.41762054125469067No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1498050.4049743025618284No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT1416440.3829123200965764No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1395550.37726503650756626No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1134940.30681321381096865No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1125660.3043045114794218No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG1110400.30017921001612385No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1088280.2941994152344625No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1048150.28335089965634025No Hit
TCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA1019170.2755166115563157No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA968000.26168360527342205No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT963030.26034004378766906No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA900000.2433008726715701No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA883050.23871870623625555No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA871530.23560445506605943No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA774590.20939824773630167No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA764500.2066705746193504No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC744840.2013558022229914No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAGA696410.18826351193023125No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATA680690.18401385668756784No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC673070.18195390929894856No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT663440.17935058996136274No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT645060.17438184547280336No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT637720.17239759168901522No Hit
TGTTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT637670.17238407497386676No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA599750.16213299820530463No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA584810.15809420371895655No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATC563480.15232797303664036No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACC546330.1476917397407321No Hit
TTGTGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT534930.14460992868689224No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA518430.14014941268791342No Hit
CCCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC515560.1393735532383941No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC515330.13931137634871135No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGA514020.13895723841182275No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA510150.13791104465933499No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG505150.13655937314449293No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT502240.13577270032285485No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGATC497180.13440480874983468No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG489410.13230431121577013No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA458420.12392665116677908No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA458370.12391313445163064No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG453190.12251280276225428No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT431120.11654652469574145No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG428230.11576525856016275No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA410120.11086950433340483Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG405270.10955838296400802No Hit
GCTCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA398480.10772281304685251No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA391700.10588994647272668No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA391050.1057142291757972No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA385970.10434093091671769No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG381750.10320012015819098No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT377480.10204579268451586No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG374580.10126182320590747No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA372900.10080766157692055No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)