FastQCFastQC Report
Thu 28 Sept 2023
SRR9295899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34529294
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA8723722.5264692640399775No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT5575381.6146811458120167No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT4803941.3912650516399205No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4698481.3607228691093423Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA4696351.3601060015881008No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3489471.0105824926510225No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT3128960.906175492612157No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT2938040.850883310849043No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG2786180.8069032630670061No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA2766510.8012066507933815No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC2364230.684702675936554No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT2246940.6507344169851836No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1935900.5606543823340262No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1926110.5578191086096345No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1864530.5399849762349616No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC1709720.49515058141646334No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1690750.48965669555827No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1689360.48925413881905605No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT1614610.4676058537426221No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1563230.4527257348499509No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1500450.43454407147739543No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG1487780.4308747233580854No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC1381560.40011243786218165No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA1296080.37535664644634786No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA1194580.34596131620878207No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1093620.31672237492026334No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG1082620.31353667410633995No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1061860.3075243878429718No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1034800.2996875638407203No Hit
TCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA1021350.2957923205727867No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA976460.2827917651603302No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA975530.28252242863697125No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA974610.28225598820526127No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATC967410.280170802217966No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT902080.26125063547491006No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA887840.2571266009667038No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA797380.23092855590965744No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC741110.2146322482006148No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG723090.2094134910490785No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAGA720300.20860548147900157No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC715840.2073138246035381No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA690220.19989403779874562No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATA686690.19887171744664112No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT676880.19603065153895124No Hit
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT657480.19041223373985No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG654390.18951734142030244No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA653500.18925958926353953No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGA625930.18127506458718792No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT620990.1798443953125714No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA609740.1765862922074225No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCA609530.17652547428279305No Hit
TGTTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT597890.17315442360333227No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA571990.16565354623236722No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT568670.16469204380489216No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG565140.16366972345278766No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCTGTAGGCACCATCAATAGATCGGA557510.16146000552458442No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA550210.15934585862079892No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC537500.15566492613489288No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA535220.15500461723891604No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGATC530590.15366372680541918No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT507540.1469882355544252No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCAC503720.14588192854449905No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG499790.1447437645264337No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCCTGTAGGCACCATCAA495460.1434897568424075No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA482770.1398146165397995No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCCTGTAGGCACC441390.12783058929614954No Hit
TTGTGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT439080.12716159212522563No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA436350.1263709591050428No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACC434830.12593075317439156No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAA433340.1254992355186874No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG432710.12531678174479907No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT429100.1242712926595024No Hit
GCTCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA424940.12306651853350956No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTCTGTAGGCACCATCAATAG418410.12117537068669867No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA407580.11803890343080864Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG407120.11790568321495365No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGATC404360.11710636191982378No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA401050.11614775558399776No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG390750.11316478118550584No Hit
CCCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC389780.11288386029555078No Hit
GGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC389350.11275932835464288No Hit
CGTTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT377200.10924057700108204No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA376110.10892490301133873No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT375320.10869611177106604No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA374390.10842677524770705No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATC370270.10723358548831032No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA356440.10322829073771389No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGA354110.10255350138349195No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA351370.10175997227166012No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG346860.10045383493795153No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCTGTAGGCACCATCAA345690.10011499221501603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)