FastQCFastQC Report
Thu 28 Sept 2023
SRR9295900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31869106
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA7891612.4762570999010767No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT6042491.8960337324806038No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5472191.717082995676126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA4367821.3705498987012688No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT3729681.170312088453313No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT2992700.9390599158947226No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT2798210.8780321606762361No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT2796930.877630517781076No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA2693900.8453014025558169No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG2117820.6645369970528825No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1991610.6249343800230857No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1791790.5622341586864721No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1697710.5327134058922143No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT1637000.5136636088881815No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1465850.4599595608361276No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1411630.44294621882396074No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA1406970.441483987658769No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1394870.43768720716545984No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1302920.4088348132514292No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAAT1249170.3919689494898288No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGCTGTAGGCACCATCAATAGATCG1234840.3874724317651082No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1233950.38719316443956725No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCGGCTGTAGGCACCATCAATAGATC1160440.36412693848393485No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1148060.3602422986073095No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGATC1076440.3377691234890618No Hit
GGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT1045930.3281955885427097No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAA915820.2873692158167223No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCA899920.28238005797840704No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAG898230.2818497638433911No Hit
TCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA825180.2589278783032069No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA820820.2575597821915682No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA806860.2531793643662298No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC725250.22757149196466323No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATAGA701160.2200124471643478No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC676740.212349853805124No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATA668880.2098835154020323No Hit
TGTTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT663960.20833970052376113No Hit
TGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGAT659720.20700925843354376No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCA644300.2021707166809135No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA639910.2007932070639195No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA630690.19790012308472035No Hit
GCGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATA611970.19202609574300578No Hit
GGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGA605490.18999277858625843No Hit
ACCCCTCCTCCCCGCGCCCCCGCCCCTGTAGGCACCATCAATAGATCGGA600320.18837051783002634No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCA588250.18458315084207258No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGTAGGCACCATCAAT585300.1836574894821336No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA584110.18328408710303953No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT583240.1830110954477355No Hit
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCTGTAGGCACCATC565400.1774131975964434No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA562290.17643733087460942No Hit
GCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGATC559420.17553677219561792No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA542170.1701240066163136No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTATTCTGTAGGCACCATCAAT533710.1674693981061157No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTATCTGTAGGCACCATCAA531990.16692969046574446No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC529620.16618602354267484No Hit
CTGGAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA456540.14325472449713525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCAT454780.1427024655162903No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAAT450690.14141909095284944No Hit
CGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCG444670.1395301142115502No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG430300.13502104514635585No Hit
CGTTGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCAT412470.12942628513018217No Hit
GCTCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA398960.12518706988517342No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGATC397240.12464736224480223No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCTGTAGGCACCATCAATA386070.12114240041750779No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG382360.11997826358856756No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATCA379260.119005534701852No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATA375280.11775667632471397No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGCTGTAGGCACCATCAAT352460.11059613658444012No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAG340770.10692800733098694No Hit
GCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATC335180.10517395749978051No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAG333440.10462797418917243No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA332160.10422633129401244No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATA329200.10329753209895502No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA319700.1003165887364396No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG319180.10015342131028088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)