FastQCFastQC Report
Thu 28 Sept 2023
SRR9295905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45596529
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length6-61
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC17740543.8907654571689No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT10034842.2007903277023564No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC8718271.9120468577772665No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC6309231.3837083958737297No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT6060841.3292327580461223No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC5916781.297638247858735No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT5495281.205197000850657No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCT5021341.1012548784141003No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4632781.016037865513842No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG4625961.014542137626309No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC4553820.9987207578892684No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG3762280.8251242106608597No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC3745240.8213870840914229No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG3609190.7915492865695983No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC3562210.7812458707109043No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT3280540.7194714316960399No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT3247700.712269129082172No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC2879760.6315743902348356No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC2814760.6173189191659743No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2784410.6106627107515136No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC2639750.5789366116004138No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC2635030.5779014450858748No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2229180.48889247688129944No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG2204980.48358505534489254No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC2157240.47311496013216264No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2148020.47109287638977965No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1968210.43165785711451854No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1929790.4232317771381239No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1893940.41536933655629793No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1817260.39855226699383195No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1711260.37530488340461177No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1628400.3571324475159063No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC1356370.2974722045180237No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1354230.2970028705474489No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG1344290.29482288004861074No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1341960.2943118762395269No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA1235920.27105572005272593No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC1231780.2701477562031092No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1227940.26930558683534883No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1200120.2632042452178761No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA1155620.2534447304091941No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC1122810.2462490072435119No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT1106140.24259302720169773No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC1078140.2364522088951113No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1058050.23204617176013553No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG1041560.22842966840743514No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1039890.22806341245843517No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1036770.22737914984712979No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1033020.22655671882392628No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1032290.22639661891807597No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG1025970.2250105485003036No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1019710.2236376369789025No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC1005380.22049485389556733No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG981930.2153519185638012No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT977310.21433868354321442No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTC933710.20477655218010127No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC929150.20377647605588572No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG877540.1924576320272098No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC874080.19169880233646733No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC840460.18432543406977317No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCT836060.183360448335881No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC835020.18313236079877923No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC833410.1827792637461505No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGT814220.17857061005674357No Hit
TATCAGA791380.17356145683808522No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG790250.17331363095642652No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC786370.17246268899108527No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC781570.17140997728138474No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC770780.16904356908395374No Hit
CCCCCGCCCCTTGCCTCTCGGCG768140.16846457764361844No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG732450.16063722745211592No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT707520.15516970600985877No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG706420.15492845957638574No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGG696340.1527177649860146No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC684210.15005747476962555No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT671370.14724147094617662No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC669510.14683354515866767No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC649830.14251742714889548No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC647690.14204809317832065No Hit
TATCAGAT644260.1412958429357638No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCC629760.13811577631271013No Hit
CCGCGAGGGGGGCCCGGGC625040.13708060979817127No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT619130.1357844585056025No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC613620.13457603318884206No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC603070.1322622605768961No Hit
TCTCCCCCGCGGGGGCGCGCCGGC601680.131957412810962No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCC589020.12918088567662683No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG587480.1288431406697646No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGGT586810.12869619966028556No Hit
TCCCCCGCGGGGGCGCGCCGGC577710.12670043371064496No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC572470.1255512234275552No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG568870.12476168964527981No Hit
CCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT553000.12128116155508241No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC547760.12013195127199267No Hit
ACCTCTAGCATCACCAGTATTAGAGGC538720.1181493442187233No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC537530.1178883594406934No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCT535910.11753306923866946No Hit
CCGCCGGCCGAGGTGGGATCCCGAGGCCTC535540.11745192271104671No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGC527110.11560309777088515No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC524840.1151052528581726No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC524410.1150109474341786No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCG508970.1116247247679752No Hit
ACCTCTAGCATCACCAGTATTAGAGGCA508390.11149752210305307No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC505190.11079571429658604No Hit
AAGCGCCCGGCCGC502970.11030883513084955No Hit
CCCCCCGCGGGGGCGCGCCGGC490850.1076507380638557No Hit
CCCCCGCCCCTTGCCTCTCGGC485370.10644889219528092No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC482110.10573392549244265No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC475090.1041943346170056No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT468540.10275782176314342No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT464120.10178844973046085No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGC458060.10045940119696392No Hit

[OK]Adapter Content

Adapter graph