FastQCFastQC Report
Thu 28 Sept 2023
SRR9295906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29782007
Total Bases832.3 Mbp
Sequences flagged as poor quality0
Sequence length6-62
%GC71

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC10189843.4214752551767242No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC6156852.0673052692520018No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT5074121.7037535448836607No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC4828941.6214286700019915No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT4332011.4545728902689465No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT4098641.3762134969614372No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT3257131.0936569855752165No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCT3135161.0527027275226952No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC3071971.0314852185750947No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2907060.976112859015848No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC2567860.8622185872160999No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC2516100.8448389660240159No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG2477220.8317841037375352No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG2345760.7876433579509937No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC2094400.7032434046503313No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1915100.6430392686429763No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1861410.6250116051614655No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT1774660.5958832794579627No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1686160.5661673506422855No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1638760.5502517006325329No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1564020.5251560111445813No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1556910.5227686636431185No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1513760.5082800497629323No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1434950.4818177633226666No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1385740.46529436380832223No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1300210.43657568141730674No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1280320.4298971523309359No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1240950.41667776117304656No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT1195150.40129934829442493No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1148660.38568925190300307No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC1083550.3638270583980455No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG968770.3252870097035435No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC961620.32288623127380234No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC949870.3189408960920599No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC948020.3183197156591898No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG856740.28767033732817265No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC741410.24894561336984444No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC722690.24265993893561305No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG710270.23848963570520954No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA708210.2377979428988785No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC697630.23424546236927554No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC695700.23359742007984888No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA677960.22764080338843518No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG672500.22580748167845105No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC661470.2221039032057175No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG650270.21834324328780125No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC647990.21757768037593975No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC597660.20067821486980378No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT589420.1979114436444797No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC566170.190104716582734No Hit
TCTCCCCCGCGGGGGCGCGCCGGC562270.18879520107560246No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG561880.1886642495248893No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG561850.1886541763286806No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGG551750.18526286693841687No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA550280.18476928032419038No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT549110.18437642567205093No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG544400.18279493386728435No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGGT541000.18165330496363125No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGT538890.18094482349695237No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTC534570.17949428324289898No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCC511650.171798361339449No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG503330.1690047282575684No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCT500030.16789667667461095No Hit
CCCCCGCCCCTTGCCTCTCGGCG484150.1625645981481369No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT476950.16014703105804792No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG473760.1590759145278557No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG471120.15818947326148972No Hit
CCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT461680.15501977418781748No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT444890.14938214204301276No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC444330.14919410904711694No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC441420.14821700901487264No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC441100.14810956158864647No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT431630.14492978931876552No Hit
AAGCGCCCGGCCGC430410.14452014600627822No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT424630.1425793768700679No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC422990.1420287088106587No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC410700.1379020560971596No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG408330.137106273596672No Hit
TCCCCCGCGGGGGCGCGCCGGC406680.13655224780519326No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCC402060.13500097558905283No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC397920.13361087451225162No Hit
CCGCGAGGGGGGCCCGGGC390180.13101198989040597No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT383080.12862800012101266No Hit
TATCAGAT377260.12667380005652407No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC376940.1265663526302979No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC375190.1259787495181235No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC372750.12515946289314886No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG366690.12312467725899064No Hit
AAGCGCCCGGCCG365870.12284934322928606No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC360250.12096229780618882No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC358890.12050564624472757No Hit
TTGGATCGCCCCAGCTGCGGCGGGCGTCGCG357360.11999191323808366No Hit
AACGATGCCGACCGGCGATGCGGCGGCG355820.11947482249937016No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT355270.11929014723554394No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC353820.11880327608545657No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGC351340.11797055853220367No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC342970.11516013678997522No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG336440.11296753774854731No Hit
CCCCCGCCCCTTGCCTCTCGGC330550.11098983355957172No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG329550.11065406035261492No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCT323620.10866292523536106No Hit
TATCAGA316980.1064333911411679No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGT309490.10391844982106141No Hit
GGGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC304750.10232688482008617No Hit
CCGCCGGCCGAGGTGGGATCCCGAGGCCTC304100.10210863223556424No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC303280.10183329820585967No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCT300240.100812547656711No Hit

[OK]Adapter Content

Adapter graph