FastQCFastQC Report
Thu 28 Sept 2023
SRR9295907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26583487
Total Bases744.8 Mbp
Sequences flagged as poor quality0
Sequence length6-61
%GC73

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC7938152.9861206695720544No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC5620432.1142561169646403No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT4328261.6281761681603322No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT4063061.5284149893503436No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC4005091.5066082188540577No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT3887961.462547031546313No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT3776791.4207278375481742No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT3077721.1577563169195977No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG3050451.1474980690080274No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG2826861.063389464294131No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG2540570.9556947890244798No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG2418510.9097790669824466No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCT2368160.8908387375967645No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC2349500.8838193424361521No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1976680.7435743851060623No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1941710.7304196022139609No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1898510.7141689124530578No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC1870240.7035344911673928No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1809990.6808700453781703No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT1678770.6315085752294272No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG1605610.6039877311806385No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1527190.5744882151841104No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC1455980.547700909214807No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC1391840.523573149000355No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1369490.5151656740893322No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGT1356680.5103468931671755No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1334640.5020560320021222No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG1139780.4287548883259747No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT1137940.42806272931763995No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1130100.4251135300647353No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC1116150.41986591149611036No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT1086660.4087725586940494No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA1029810.3873871023767499No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC981290.36913517026566156No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC979300.36838658525121254No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT964400.36278160197719733No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC888210.33412095260490093No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC878510.33047207087617964No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT862440.3244269647544733No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC832540.31317938086903346No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG830550.31243079585458444No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT793600.298531189681775No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC778460.292835924797977No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT726780.2733952848247485No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC678230.25513206751243733No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC675130.2539659300527429No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGT666370.2506706512956709No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC654170.24608133613171213No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG646550.2432148950211084No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGGT595810.22412785801952917No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG579760.21809027536530476No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC551520.2074671392808626No Hit
TCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGG547840.20608282126419308No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA546790.20568783922139336No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG534440.20104209805132034No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT530140.19942455254271194No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG528770.19890919502020182No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG524980.19748349793238187No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC517590.19470357669781996No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC508080.19112616791017673No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA494960.1861907732420506No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG487880.18352746575345816No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC486750.18310238984073082No Hit
TCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG477440.17960021572790658No Hit
CCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT465670.17517265511480867No Hit
CCGCGAGGGGGGCCCGGGC464090.17457830118373863No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGT438220.16484669599590152No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGG436480.16419215432497627No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGT434890.16359403866016525No Hit
TCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG429470.16155517897257046No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC428710.16126928720825828No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC423890.15945613154512045No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCC419900.15795519978248151No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC408480.15365929985031684No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC405090.15238407211213487No Hit
TTGGATCGCCCCAGCTGCGGCGGGCGTCGCG403590.1518198120509924No Hit
TCTCCCCCGCGGGGGCGCGCCGGC398210.14979599929836143No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC396600.1491903601660685No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTC392280.14756529118997821No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG391690.14734334889926218No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC391290.14719287954962418No Hit
AAGCGCCCGGCCGC390570.1469220347202758No Hit
ACGATGCCGACCGGCGATGCGGCGGCG385430.1449885035774276No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG378760.1424794271722141No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCG374820.14099730407827987No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC371230.1396468416652789No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC368360.1385672240816263No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG357540.13449702817391865No Hit
TATCAGA356470.134094522663637No Hit
AAGCGCCCGGCCG356280.13402304972255896No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCT356260.13401552625507707No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG352310.1325296414274019No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG350490.13184500588654904No Hit
AAAACGATGCCGACCGGCGATGCGGCGGC349380.13142745344130363No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC348860.13123184328677423No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC347130.1305810633495899No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG346080.13018608130679019No Hit
CGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG335730.12629268688490716No Hit
TTTGGTCTAGGGGTATGATTCT333380.12540867945578396No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTC329150.12381746608336221No Hit
CCCCCGCCCCTTGCCTCTCGGCG321800.1210525917837641No Hit
GTATAGTGGTGAGTATCCC317850.11956670695608895No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC313070.11776859822791494No Hit
TCTCCCCCGCGGGGGCGCGCCGGCG310120.11665888677433475No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC298030.11211095068152646No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCG297880.11205452467541223No Hit
TCCCCCGCGGGGGCGCGCCGGC297660.11197176653311132No Hit
TATCAGAT290650.10933479118070553No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTAT282580.10629907205175905No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT281610.10593418387888692No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC281040.10571976505565278No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC280470.10550534623241864No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGTT274150.10312793050813837No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGG270070.10159314314183088No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG269660.10143891205845193No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGC268570.1010288830806884No Hit

[OK]Adapter Content

Adapter graph