FastQCFastQC Report
Thu 28 Sept 2023
SRR9295908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9295908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19997171
Total Bases545.7 Mbp
Sequences flagged as poor quality0
Sequence length6-61
%GC72

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCGGCCGAGGTGGGATCCCGAGGCCTC5541072.7709269476167404No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC4262872.131736534132753No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT3437791.7191381720944428No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC3252851.6266550903625319No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGT3035601.5180147231825942No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCT3005831.5031276174014814No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCT2689211.3447952212840506No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT2447261.223803106949478No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG2189041.0946748417563665No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG2170871.085588556501317No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCCT2014851.0075675204257644No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTC1912870.9565703068699067No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG1634900.8175656446604371No Hit
AACGATGCCGACCGGCGATGCGGCGGCG1620880.8105546529556605No Hit
ACGATGCCGACCGGCGATGCGGCGGCGT1605290.8027585501969253No Hit
AACGATGCCGACCGGCGATGCGGCGGCGT1480020.7401146892227906No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG1375450.687822292463269No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCC1309840.6550126515395602No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGC1273900.6370401093234638No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC1157940.57905190689223No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGG1094850.547502444220735No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGCC1055240.5276946424071685No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT1017930.5090370032841145No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC867830.43397638595979404No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC850650.4253851707324001No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCG828670.41439361597698No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCT792700.39640607163883335No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC762830.38146895878422005No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGT703920.352009791785048No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC652770.3264311736895184No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC622840.3114640565908047No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC586790.2934365065938577No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCC558690.2793845189402041No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC551020.2755489764027122No Hit
ACGATGCCGACCGGCGATGCGGCGGCG549490.2747838681781538No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTA490660.2453647068377822No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCC488740.2444045710265717No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTT487380.24372447482696427No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG485900.24298437013915616No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCC483350.24170918976489222No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCC473660.23686350434268927No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT457750.2289073789487523No Hit
TCACCCCCGCGGGGAATCCCCCGCGAGGGGGGTC451190.22562691492711645No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTC438080.21907098759119478No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC432470.21626559076781413No Hit
ACCCCCGCGGGGAATCCCCCGCGAGGGGGG428710.21438532480419353No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC414090.20707429065841362No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCG400710.2003833442240405No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT399010.1995332239745312No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT395650.19785298630491283No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA366040.18304589184140097No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGC363790.18192073268763867No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCC359310.17968041579481417No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCT356100.17807518873544662No Hit
TAACGATGCCGACCGGCGATGCGGCGGCG347970.17400961365985218No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG347170.1736095570718478No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCA342330.17118921471442136No Hit
GGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC341140.17059413053976485No Hit
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGC340120.17008405839005927No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCG338810.16942896572720212No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGG334910.1674786898606808No Hit
CACCCCCGCGGGGAATCCCCCGCGAGGGGGGT332960.16650355192742014No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCCT331910.1659784776556644No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGG329950.16499833901505367No Hit
CCGCGAGGGGGGCCCGGGC325850.16294804900153126No Hit
TAACGATGCCGACCGGCGATGCGGCGGCGT323390.16171787499341783No Hit
TCTCCCCCGCGGGGGCGCGCCGGC309830.1549369158267437No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGC308620.15433183023738709No Hit
CCACCCCCGCGGGGAATCCCCCGCGAGGGGGGT301210.1506263060909966No Hit
TAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG295810.14792592412196703No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC293420.14673075506530397No Hit
TTCGCCGGCCGAGGTGGGATCCCGAGGCCTC291200.14562059803359184No Hit
CGCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG289550.14479548132083284No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCC284640.14234013401195597No Hit
TATCAGAT283870.14195507954600178No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCT282550.14129498617579458No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC279880.13995979731332997No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC276700.1383695723760126No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCG271140.1355891790893822No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGC270640.13533914372187947No Hit
AAGCGCCCGGCCG268070.1340539619329154No Hit
TATCAGA266520.13327885229365694No Hit
CCCCCGCCCCTTGCCTCTCGGCG265230.1326337610454999No Hit
AAGCGCCCGGCCGC263260.1316486216975391No Hit
TCCCCGCGAGGGGCCCGGGGCGGGGTCCGCCGG262410.13122356157278448No Hit
TCGCGCGGGTCGGGGGGCGGGGCGGACTGT253960.12699796386198828No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGC247680.12385751964615395No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGC247410.12372250054770247No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG244770.12240231380728804No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTC234660.11734659867638278No Hit
TCTCGCCGGCCGAGGTGGGATCCCGAGGC230920.11547633412746232No Hit
TCCCCCGCGGGGGCGCGCCGGC230080.11505627471005773No Hit
AAACGATGCCGACCGGCGATGCGGCGGCG228510.11427116365609914No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGT228400.11421615587524855No Hit
TTTGGTCTAGGGGTATGATTCT227040.11353605967564112No Hit
TCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCG226710.11337103633308931No Hit
TTGGATCGCCCCAGCTGCGGCGGGCGTCGCG222370.11120072934316559No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGG217510.10877038557103903No Hit
CCTCGCCGGCCGAGGTGGGATCCCGAGGCC217040.10853535232558645No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGT216680.10835532686098448No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCG214210.107120152145521No Hit
CCTCGCTTCTGGCGCCAAGCGCCCGGCCG213840.10693512597356897No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCC213610.10682010970451769No Hit
GTATAGTGGTGAGTATCCC213010.10652006726351443No Hit
AAGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCG212610.10632003896951224No Hit
AAAACGATGCCGACCGGCGATGCGGCGGC212220.1061250113828601No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGC211030.10552992720820359No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGC207140.10358465204903233No Hit
AACGATGCCGACCGGCGATGCGGCGG206490.10325960607127879No Hit
TCTCCCCCGCGGGGGCGCGCCGGCG205120.1025745091643213No Hit
TTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC202360.10119431393570621No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC201210.10061923259044993No Hit

[OK]Adapter Content

Adapter graph