FastQCFastQC Report
Tue 27 Jun 2023
SRR931815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR931815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93826407
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length26-36
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT20840402.2211657321589646No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT16963971.8080165853521388No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT12105181.2901677030007128No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT9805881.0451087613319776No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT8209430.8749594343946262No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT6081950.6482130345244916No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC5746150.6124235365849616No Hit
GGGGGGGGGATGCGTGCATTTATCAGA5104810.5440696455529838No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCT4349100.46352622242051744No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT4154230.4427570161564431No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4126920.4398463217290202No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGG4106560.43767635693435436No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT4030690.42959014725992867No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT3926960.4185346242662793No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC3743890.3990230596808423No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3739720.3985786219011882No Hit
AATACATGCCGACGGGCGCTGACCCC3690610.39334448776238445No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGAT3649250.3889363470989569No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCT3491390.3721116593540665No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACC3299640.351674982076208No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA3207560.341861113790705No Hit
AAATTTTTGACAGTCTCTACGGAGACTG3136900.33433018489133876No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3084350.32872941622927115No Hit
CTTTGGTGACTCTAGATAACCTCGGGCC3075550.32779151396045675No Hit
AAATACATGCCGACGGGCGCTGACCCC3073410.32756343318144965No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCC3035790.32355390098226827No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCC3020560.32193069057839974No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2945120.313890310219382No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2936700.31299290827581194No Hit
AGTTGGATCTTGGGAGCGGGCGGGCG2628520.2801471445027198No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGA2494170.26582814793280957No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTT2437830.25982344181633216No Hit
AAATACATGCCGACGGGCGCTGACCC2378360.253485140915606No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT2338370.24922301458266435No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCT2315010.2467333103781753No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG2309220.24611621331721675No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCT2103750.2242172611384341No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGAT2093980.2231759764604436No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGC2081300.2218245445549247No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTT2068200.2204283491320306No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2028750.2162237758928571No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2019150.21520060978142325No Hit
AGATCAGACGTGGCGACCCGCTGAATTT2006810.21388541500901767No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC1940460.2068138450617639No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTT1881250.20050325490988907No Hit
TAAGGTGAACAGCCTCTGGCATGTTGGAAC1873970.19972735394205174No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATC1846630.19681346212053075No Hit
ACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTC1828340.19486411751864272No Hit
GGGGGGGGATGCGTGCATTTATCAGA1809570.19286361461118298No Hit
GGGGGGGGGATGCGTGCATTTATCAGAT1793410.1911412849902693No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA1787450.19050606936275413No Hit
TAAGGGAAGTCGGCAAGCCGGATCCGT1773840.1890555182401901No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTCG1765150.18812933974973592No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGT1716180.18291012678339053No Hit
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1713510.182625558708648No Hit
TTTTGGTGACTCTAGATAACCTCGGGCC1699100.18108974374346445No Hit
GAGAAGACGGTCGAACTTGACTATCT1626170.17331687869066542No Hit
TCACCCCTCCCACGGCGCGACCGCTC1625910.17328916794181407No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTT1612610.17187165655826508No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC1590730.1695396904626221No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTC1580560.1684557738633219No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1567170.1670286702974782No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATT1554040.16562927748048584No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT1544310.16459225599462635No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGG1503370.16022887884857406No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGT1500330.15990487624662No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA1479140.15764645021523632No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT1477290.15744927757917873No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC1472690.1569590104841167No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTT1433470.15277895059969632No Hit
TGATGCCGACCGGCGATGCGGCGGCGTT1423610.15172807373941113No Hit
GCGGGGCTACGCCTGTCTGAGCGTCGCT1382370.14733272265237654No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT1376480.14670496761109056No Hit
TTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1363010.14526933766098493No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1350180.14390191878497488No Hit
AGGAGGTGTCAGAAAAGTTACCACAGGGAT1338440.14265067189453393No Hit
GATGCCGACCGGCGATGCGGCGGCGTT1330500.1418044282565355No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1315270.140181217852667No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATT1311230.13975063544743857No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG1305030.13908984066713756No Hit
GATGCCGACCGGCGATGCGGCGGCGTTA1304400.1390226953910747No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTC1299100.1384578224337206No Hit
TGATGCCGACCGGCGATGCGGCGGCGTTA1250040.13322901728508052No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1241120.13227832543987322No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTC1199550.1278478030177581No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGC1185100.12630772486044362No Hit
TATCCGGTAAAGCGAATGATTAGAGGTC1183450.1261318681850409No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGA1159780.12360912424153683No Hit
AGTTTTATCCGGTAAAGCGAATGATT1150180.12258595813010296No Hit
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1125230.11992679203840768No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGAC1109590.1182598839151967No Hit
AGGTAAGGGAAGTCGGCAAGCCGGATCCGT1072250.11428019406093212No Hit
TGAGAAGACGGTCGAACTTGACTATCT1050120.11192158301447054No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1045370.11141532894891734No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC1023730.1091089420060602No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG1012920.10795681433266437No Hit
TAGTCAGCGGAGGAGAAGAAACTAACC1011930.10785130032742274No Hit
CTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1003320.10693364822123051No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGG1002300.10682493682189066No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGT998590.1064295257517428No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTA994040.10594458764684445No Hit
AGAATCCCGCCCAGGCGGAACGATAC994040.10594458764684445No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT988280.10533068797998414No Hit
AATACATGCCGACGGGCGCTGACCCCC982720.1047381042737787No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTA982360.10469973554459992No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTT978300.10426702154330604No Hit
TGACCGGGGTCCGGTGCGGAGTGCCCTTCGTC972140.10361048995513597No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTT969120.1032886189492474No Hit
GGCGGCGACGACCCATTCGAACGTCTGCCCT966070.10296355054926062No Hit
AACTTCTTAGAGGGACAAGTGGCGTTC954450.10172509323521256No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTC948890.10113250952900711No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCC946660.10089483656770529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)