FastQCFastQC Report
Sun 2 Jul 2023
SRR931817.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR931817.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127399109
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length26-36
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT19736611.5491952930377244No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT16984681.3331867179699035No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT16469171.2927225417251544No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT16274501.2774422150785998No Hit
AATACATGCCGACGGGCGCTGACCCC16176461.2697467138486815No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT15993981.2554232227793682No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT12873071.010452121764839No Hit
AAATACATGCCGACGGGCGCTGACCC12444760.9768325773769737No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGC12169250.9552068374355743No Hit
AAATACATGCCGACGGGCGCTGACCCC11275300.8850375868798267No Hit
TGATGCCGACCGGCGATGCGGCGGCGTT10161860.7976398013898198No Hit
AGGACGGTGGCCATGGAAGTCGGAATCCGCT10010030.7857221356234132No Hit
GATGCCGACCGGCGATGCGGCGGCGTT9801910.7693860716090252No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGG7676010.6025167727036459No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC7582240.595156438652958No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT6810980.5346175537224518No Hit
TAAGGGAAGTCGGCAAGCCGGATCCGT6483540.5089156471259152No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC5878070.46139019700679385No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT5637550.4425109440914536No Hit
TAAGGTGAACAGCCTCTGGCATGTTGGAAC5270880.41372973809416513No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5252840.41231371563203No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT5244340.41164652101295307No Hit
AAGTGGGAGGCCCCCGGCGCCCCCCCGG5214730.4093223289340273No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTT4955640.3889854520097154No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGC4942480.38795247775241504No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT4916990.3859516788300301No Hit
AGAACTGGTGCGGACCAGGGGAATCC4748780.3727482897859199No Hit
TGCTGAGAAGACGGTCGAACTTGACT4479110.35158095179456866No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4145730.32541279389952404No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTT4134840.3245579998522595No Hit
AGGTGTCCTAAGGCGAGCTCAGGGAGGAC3956690.31057438557125233No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCTT3914480.3072611755864007No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACC3774320.2962595287852445No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3756630.2948709790427184No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCT3703040.2906645132031496No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT3678970.2887751750288929No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG3670180.28808521729928266No Hit
AATACATGCCGACGGGCGCTGACCCCC3471010.2724516699720404No Hit
AACGGCGAGTGAACAGGGAAGAGCCC3460200.2716031554035437No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT3436270.2697248063171305No Hit
AAATTTTTGACAGTCTCTACGGAGACTG3414940.2680505402906703No Hit
TGAAAGTCGGAGGTTCGAAGACGATC3391340.2661980940541743No Hit
TGAAGAACGCAGCTAGCTGCGAGAATT3385450.26573576742989624No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3227110.2533071090787613No Hit
AGATCAGACGTGGCGACCCGCTGAATTT3199670.25115324786141163No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCT3122340.2450833466975032No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3065070.2405880248346164No Hit
GATGCCGACCGGCGATGCGGCGGCGTTA2825900.22181473812348246No Hit
TGATGCCGACCGGCGATGCGGCGGCGTTA2548600.2000484948446539No Hit
AAAGGGAAGTCGGCAAGCCGGATCCGT2506830.1967698219930251No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATC2480420.19469680906481066No Hit
AGTTGGATCTTGGGAGCGGGCGGGCG2479000.19458534831668248No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTT2478060.19451156444116105No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCC2465870.19355472886392008No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTT2452590.19251233538846807No Hit
TATGGTGACCACGGGTGACGGGGAATC2438200.19138281414511304No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2350760.184519343851926No Hit
TGGCCGATCGAAAGGGAGTCGGGTTC2320340.18213157205047645No Hit
TAGCCTCTGGCATGTTGGAACAATGT2304230.1808670420136141No Hit
TGATGAAGAACGCAGCTAGCTGCGAGAATT2300750.18059388468721552No Hit
GCGGGGCTACGCCTGTCTGAGCGTCGCT2300400.18056641196760648No Hit
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTT2297900.18037017825611323No Hit
TCGGCCGATCGAAAGGGAGTCGGGTTC2252570.17681206859931806No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCT2139200.16791326225052328No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2125650.16684967553423No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2109790.16560476886851697No Hit
GAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCG2097810.16466441692304143No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTC2089710.16402861969780339No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2052500.16110787713593822No Hit
AAATACATGCCGACGGGCGCTGACCCCC1999880.1569775499764288No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGAT1952460.1532553889368253No Hit
AAGGGAAGTCGGCAAGCCGGATCCGT1938440.15215491028277128No Hit
AGAATCCCGCCCAGGCGGAACGATAC1924500.15106071110748506No Hit
GGGGCTACGCCTGTCTGAGCGTCGCT1919140.15063998603004358No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT1904440.14948613180646342No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGG1899400.1490905246440931No Hit
TGGCCCTTGAAAATCCGGGGGAGAGGG1882110.14773337229540592No Hit
GCGTCGATGAAGAACGCAGCTAGCTGCGAGAATT1853200.14546412565569827No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGACC1847950.14505203486156248No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTT1847610.1450253470767994No Hit
AAGGTGAACAGCCTCTGGCATGTTGGAAC1832940.14387384765775715No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG1812280.14225217226597717No Hit
ACTTGGATGGGAGACCGCCTGGGAAT1794830.14088246095975446No Hit
ATGAGAGGTGTAGAATAAGTGGGAGGCCC1761710.13828275674989218No Hit
AACTGGCTTGTGGCGGCCAAGCGTTC1733930.13610220774777945No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA1723380.13527410148527805No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1721420.13512025425546736No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC1702640.13364614661473026No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGG1645700.12917672760176052No Hit
AGGAGGGCCGCTGCGGTGAGCCTTGAAGCCT1626250.12765002932634326No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGAT1595010.12519789286752392No Hit
GAAAGTCAGCCCTCGACACAAGGGTTTGT1573140.12348124035938117No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCC1558100.12230069835103792No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTC1552820.12188625275236423No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGGT1552500.12186113483729308No Hit
TGAAAGATGGTGAACTATGCCTGGGC1548440.1215424512898281No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCC1537690.12069864633040721No Hit
CTTTGGGTTCCGGGGGGAGTATGGTTGC1537070.12064998036995692No Hit
ACCATGGTGACCACGGGTGACGGGGAATC1523530.11958717858850959No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGA1505430.11816644651729863No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT1498420.11761620719027163No Hit
TTAGCCTCTGGCATGTTGGAACAATGT1497290.1175275095526767No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC1479960.11616721746460566No Hit
GAGAAGACGGTCGAACTTGACTATCT1479160.11610442267692782No Hit
TAACGGCGAGTGAACAGGGAAGAGCCC1473740.11567898799041051No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG1457650.1144160278232401No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCC1428050.11209262067916033No Hit
GGGGGGGGGATGCGTGCATTTATCAGA1418240.1113225995952609No Hit
TAGTCAGCGGAGGAGAAGAAACTAACC1411120.11076372598492819No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1410940.11074959715770068No Hit
ATGATTAAGAGGGACGGCCGGGGGCATTCGT1403150.11013813291268779No Hit
GGAGCGCTGAGAAGACGGTCGAACTTGACT1391320.10920955498990184No Hit
AGGTAAGGGAAGTCGGCAAGCCGGATCCGT1389390.10905806256462909No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC1378170.10817736566744748No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1350030.10596855901087975No Hit
AGTTTTATCCGGTAAAGCGAATGATT1344540.10553762978044061No Hit
TAAACGATGCCGACCGGCGATGCGGCGGCGTT1335460.10482490894029722No Hit
TGGACGGTGTGAGGCCGGTAGCGGCCCCCG1331370.10450387058829431No Hit
AGTCAGCGGAGGAGAAGAAACTAACC1322460.10380449364053244No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGT1321040.1036930328924043No Hit
TGAAGGCCGAAGTGGAGAAGGGTTCC1284410.10081781655160554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)