FastQCFastQC Report
Tue 27 Jun 2023
SRR931819.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR931819.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67026629
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length26-36
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT17821252.658831313148689No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT12038811.796123448189525No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT11811511.7622115532619134No Hit
ACTTCGAACGCACTTGCGGCCCCGGG9255321.3808422321224No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGAT8382941.2506879914846978No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT7393021.1029974370335707No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT5729700.8548393504915786No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC5644030.842057863300868No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCC5616330.8379251774693905No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTT5596310.8349383048937162No Hit
ACTGACCCGGTGAGGCGGGGGGGGGAGCCCC5146940.7678948019301403No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGC5110680.7624850117406322No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT4592110.68511725392008No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG4567330.6814202158369026No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT4526850.6753808251344402No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT3915120.5841141138099009No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT3820300.5699674975448937No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC3760810.5610919206454498No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3501050.5223371743788576No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTT3440110.5132452655496071No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3247870.4845641274902845No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3114260.4646302591168654No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCT2727280.4068949969123466No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCT2638190.39360326475616125No Hit
AAATTTTTGACAGTCTCTACGGAGACTG2364620.3527881433512045No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2252940.336126108923067No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAAT2222050.33151749284601495No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT2207340.32932284271673573No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGA2196640.3277264622691975No Hit
ACTGACCCGGTGAGGCGGGGGGGGGGGCCCC2066290.3082789677517573No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG2042680.3047564871567687No Hit
ACCGCAGCTAGGAATAATGGAATAGG2019860.3013518701649161No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTT1997230.2979756001155899No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC1940130.2894565979142409No Hit
TGGGGCTACGCCTGTCTGAGCGTCGCT1814640.2707341883477386No Hit
GTGGCGTACGGAAGACCCGCTCCCCGGCGC1705770.25449139028012285No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA1680750.2507585455327016No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG1647590.24581125808967658No Hit
GCGGGGCTACGCCTGTCTGAGCGTCGCT1631470.24340624380796475No Hit
TAGCGCCGCGGAGCCTCGGTTGGCCTCGGAT1503440.22430488037821508No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT1491560.22253245049814455No Hit
TACTGACCCGGTGAGGCGGGGGGGCGAGCCCC1401090.20903483002255716No Hit
TACTGACCCGGTGAGGCGGGGGGGGGAGCCCC1400500.208946805306291No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCC1387990.2070803829325804No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1379650.2058361013501067No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTT1333420.19893884265013537No Hit
TCACCCCTCCCACGGCGCGACCGCTC1314840.1961668100599241No Hit
TAAGGTTTCCGTAGGTGAACCTGCGGAAGGATC1285120.19173275147106084No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1277940.19066153543243236No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCG1274680.19017516157645342No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC1262310.18832962642355175No Hit
CTTTGGTGACTCTAGATAACCTCGGGCC1187640.17718927801068438No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1171820.17482902205927736No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1163980.17365933769397832No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCT1138730.16989217822665675No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1096130.16353649532337364No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA1092820.16304266174567722No Hit
AGTTGGATCTTGGGAGCGGGCGGGCGG1079000.16098079466296897No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGG1062560.15852803816226532No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1048750.15646766302390053No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGA1037380.1547713223053482No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTT1010050.15069383841457998No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTC972990.14516469267759236No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC968760.14453360022029452No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA937790.13991304858849457No Hit
CGCTTCTGGCGCCAAGCGCCCGGCCG933230.13923272196786146No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACC922900.1376915434610325No Hit
TCTCGCCCGTCACGCACCGCACGTTCGTGGGGA919130.13712908044353536No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG913490.1362876238338049No Hit
AAGGTTTCCGTAGGTGAACCTGCGGAAGGATC878350.13104493141076812No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC878270.13103299585602016No Hit
CGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA857300.12790438856771388No Hit
AATACATGCCGACGGGCGCTGACCCC851560.1270480125145485No Hit
GCGGGGCTACGCCTGTCTGAGCGTCGCTT846860.12634679867310647No Hit
TACCCCTCCCACGGCGCGACCGCTCT844260.1259588931437981No Hit
GGGGGCTACGCCTGTCTGAGCGTCGCTT835810.12469820017354594No Hit
TCCTCGCCCGTCACGCACCGCACGTTCGTGGGGA826750.12334649859834068No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG820800.12245879171396193No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT803200.11983296966941302No Hit
TCTGGATACCGCAGCTAGGAATAATGGAAT781360.11657456322322281No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC779840.11634778768301178No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC773540.11540786274661075No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA767860.11456043835950634No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGG758090.113102808735913No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT745010.11115134553462327No Hit
AGTTTTATCCGGTAAAGCGAATGATT744940.1111409019242188No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTT722020.10772136548893127No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTA713780.10649200334989248No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA711610.10616825142735434No Hit
AGAATCCCGCCCAGGCGGAACGATAC707700.10558490118904831No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG706330.10538050481398967No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCG693550.10347379994300474No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAG683190.10192814560314528No Hit
AGATCAGACGTGGCGACCCGCTGAATTT682870.1018804033841535No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTA681060.10161036145798112No Hit
CCGCTGAGAAGACGGTCGAACTTGACTATCT679950.10144475563585331No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)