FastQCFastQC Report
Tue 27 Jun 2023
SRR931821.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR931821.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90077819
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length26-36
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT29720083.2993782853468065No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT23728162.6341845599081393No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT14695111.6313794187223827No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT8616310.9565406995477989No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC7571480.8405487703915211No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG7495810.8321482561650387No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCC7436430.8255561782640407No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT7291410.809456765377501No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5699150.6326918283845216No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA5665590.6289661609147086No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA5441140.6040488169457122No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5359240.5949566785137194No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC5325990.5912654257315No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT5033760.5588234768428396No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC4851060.5385410141868554No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG4416980.4903515703460804No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGA3611580.40093999167542016No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGAT3257530.36163508798986355No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGA3051040.3387115755988719No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2928560.32511444354575236No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2821850.3132680199550568No Hit
GGGGGGGGGATGCGTGCATTTATCAGA2763650.3068069398971572No Hit
TCTCGCCCGTCACGCACCGCACGTTCGTGGGGA2622190.29110274084233767No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2552430.28335832598255956No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC2452150.2722257296216286No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2435690.27039842072552844No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGG2414060.2679971636524637No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA2377270.2639129173409494No Hit
TCCTCGCCCGTCACGCACCGCACGTTCGTGGGGA2332720.25896719368838184No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA2318480.25738633836150054No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAA2281050.2532310423723736No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2090210.23204491662925367No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2053200.22793624699106005No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCT2010870.22323697690771133No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCC1934510.21475986224755286No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGG1929440.2141970155827152No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG1926080.21382400477524882No Hit
TGTGCATTTATCAGATCAAAACCAACCCGG1896340.21052241506868632No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1896170.21050354249807046No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1890700.2098962897847249No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC1887060.209492194743303No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1872900.2079202206261233No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG1866180.20717419901119055No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC1850280.20540905858300146No Hit
CTTTGGTGACTCTAGATAACCTCGGGCC1804670.20034565890188794No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT1796360.19942312324413625No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGA1786320.19830853142658791No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1762640.19567969335492014No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGA1755910.1949325615887747No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG1680940.1866097579471812No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG1669220.1853086607258997No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAG1628120.18074593924171276No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1608160.17853007742116847No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1556830.17283167124639195No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTA1544850.1715017100935803No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC1506990.1672986776023074No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTA1492960.16574113545089275No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGAT1399880.155407847963104No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACA1394490.15480947645946003No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA1377790.1529555239342551No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAT1377110.15288003365179167No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA1355550.15048654763721575No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT1318740.14640008102327612No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGC1314700.1459515799333463No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1298490.1441520248175636No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG1267790.1407438605945821No Hit
ATCACCTCTAGCATCACCAGTATTAGAGGCA1256010.13943610246602442No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGG1234310.13702707433447073No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC1205330.13380985612007323No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGG1163810.12920050828495303No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC1152150.1279060719709477No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCC1138530.12639404601925364No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1058350.11749285359584473No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG1053730.11697996373557845No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATC1048900.11644376069984554No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCG1015110.11269255975214054No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1009850.11210862021426163No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT999590.11096960507003394No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCG986430.10950864607412397No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG986190.10948200244501924No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACA975750.108323004578963No Hit
GGGGGGGGATGCGTGCATTTATCAGA942610.10464396346008334No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGA919280.10205398068085995No Hit
CGGGCCGCCGGTGAAATACCACTACTCTG917100.1018119677164919No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCG912130.10126022256378121No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGC910710.10110258109157816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)