FastQCFastQC Report
Mon 3 Jul 2023
SRR931823.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR931823.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93512729
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length26-36
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT44808634.791714505519351No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT44320104.739472419845645No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT16604671.7756587982797507No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT13384621.4313153025402563No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG12863261.3755624648704243No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCC12572311.3444490535614675No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT8995540.9619588794162985No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA8914590.953302303903461No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT8510520.9100921437123282No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC8085470.8646384386878496No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT7724450.8260319298349212No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA7368010.7879151938769747No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA7194000.7693070319870571No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT6999490.7485066551741849No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC5801700.6204182106587863No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC4640800.49627468363157273No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA4510060.4822936992887888No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4428300.4735505045521664No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCG4202960.4494532503697972No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG4026340.43056598209212776No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4013370.42917900513843416No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3997770.4275107830507224No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGG3790010.4052934868364284No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG3539300.3784832330152615No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG3515490.3759370555852348No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG3505460.3748644743326869No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCG3243070.3468051926920024No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGA3123280.33399517193001604No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2907360.31090526723907286No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT2784830.29780223823860386No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC2655410.28396241114939547No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTC2524290.2699407906275519No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2514450.26888852746453373No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT2509670.26837736710688875No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC2406010.257292245208671No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2395830.25620362335912583No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT2278770.24368554146248902No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCT2265270.24224188773273853No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG2208270.23614646087379182No Hit
GGGGGGGGGATGCGTGCATTTATCAGA2172500.23232131317651952No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGA2060480.22034219533898963No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2008380.21477076131528575No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA1891040.20222273697092083No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAA1814490.1940366856366688No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1769130.18918600910470704No Hit
AAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC1723780.18433640194587841No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC1721320.18407333615512386No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGA1696070.1813731689939238No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1678310.179473962309452No Hit
TTGCGTGCATTTATCAGATCAAAACCAACCCGG1640300.17540927503035442No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG1609050.17206748398926525No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT1602240.1713392408855911No Hit
TCCTCGCCCGTCACGCACCGCACGTTCGTGGGGA1581000.16906789235078362No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG1579220.16887754393308316No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA1569340.1678210032775324No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC1525280.16310934525287996No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1520770.16262705796982999No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1500600.16047013236026939No Hit
CCATGACCCGCCGGGCAGCTTCCGGGAAACC1497270.1601140311069309No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA1495520.1599268908086299No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCC1481080.15838271600436343No Hit
GAGAAGATTAGCATGGCCCCTGCGCAAGGATGACA1473080.1575272174978446No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCT1470280.15722779302056303No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCG1456420.15574564185801915No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1447490.1547906916501175No Hit
TGTGCATTTATCAGATCAAAACCAACCCGG1440740.15406886478524223No Hit
TTGGATGGTTTAGTGAGGCCCTCGGATCGGCC1439950.1539843843077235No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGC1426220.15251613499591057No Hit
TCTCGCCCGTCACGCACCGCACGTTCGTGGGGA1412830.15108424437062468No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAG1410400.15082438669926956No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA1410290.15081262359480493No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1377940.14735320150906941No Hit
TCGCCGCTGGTGTAGTGGTATCATGCAAGATT1373320.1468591511215548No Hit
CCCTCGCCCGTCACGCACCGCACGTTCGTGGGGA1369290.14642819374889593No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT1350480.14441670288544353No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGT1282490.13714603495316663No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1278200.13668727387904592No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTC1265590.1353387943581456No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGA1258900.13462338373206925No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGA1255860.13429829429959209No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAG1239060.13250174743590254No Hit
AGTTTTATCCGGTAAAGCGAATGATTAGAGGTC1231340.13167619137711187No Hit
AGAGCTAATACATGCCGACGGGCGCTGACCCC1224820.13097896009429902No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAG1209380.12932784797671767No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC1184560.12667366386024304No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCT1179580.12614111603993505No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT1169410.12505356356352298No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCT1167300.12482792583242866No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGG1162690.12433494481804719No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAATC1119290.11969386542018254No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA1118550.11961473180832954No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGA1094280.11701936321417805No Hit
TGGGCCGCCGGTGAAATACCACTACTCTGAT1091100.11667930255783682No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGG1080740.11557143199189492No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG1074010.11485174387328596No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGT1062440.11361447915823311No Hit
GGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1057200.11305412763646326No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG1041480.11137307307115378No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCC1040400.11125758077277373No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA1025250.10963748047605369No Hit
TATCGACACTTCGAACGCACTTGCGGCCCCGGGT1016110.10866007343235592No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGC1000570.10699826758344311No Hit
CCCCCGCCACGCAGTTTTATCCGGTAAAGCG981320.10493972430213216No Hit
CGGGCCGCCGGTGAAATACCACTACTCTGAT966960.10340410448293087No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGG952770.1018866640069931No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTT944450.1009969455602135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)