FastQCFastQC Report
Thu 29 Jun 2023
SRR931825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR931825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201296355
Total Bases6.3 Gbp
Sequences flagged as poor quality0
Sequence length26-36
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT56081052.78599431172015No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT50844832.525869382980134No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT30718141.5260157095243976No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA29235061.4523392636692305No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGG24464311.2153379528407258No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT20720281.0293420365212276No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGGA15779540.7838959627460717No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG15291510.7596516091908371No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCC14893900.7398991402502048No Hit
GGGGGGGGGATGCGTGCATTTATCAGA14681400.7293425655919105No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGA14652380.7279009100785754No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGG13557610.6735149277789952No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT12386010.6153121848629599No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG12326040.6123329952993933No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC10630690.5281114007255621No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT9415730.4677546197992507No Hit
ATGTACACGGAGTCGACCCGCAACGCGA9375630.4657625320637326No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGG8819280.4381241776583585No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC8656330.4300291478203865No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGGG8404450.4175162535854164No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAA7854960.39021869024901124No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGA6961490.3458328890257352No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCG6900700.34281296350348717No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC6728040.33423556030112916No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT6498140.32281458847081457No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATA6295250.31273541937706717No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTCGGAT6012820.2987048622912223No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT5981480.29714795382161785No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5895950.2928989946191524No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATA5843130.2902750027440884No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT5797140.28799031159804156No Hit
TTGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT5519330.27418926686476763No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCT5351570.2658552858545302No Hit
GGGGGGGGATGCGTGCATTTATCAGA5210740.2588591333409887No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5195490.2581015438655111No Hit
TGGGCCGCCGGTGAAATACCACTACTCT5157000.2561894377074041No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTG5116840.25419436929198247No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCT4966880.2467446566531222No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAA4957090.24625830904886478No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG4731940.2350733077109121No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4703700.23367040103632278No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTC4410370.21909835376800538No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGGA4331180.21516435307534507No Hit
TTCTCGCTTCTGGCGCCAAGCGCCCGGCCG4285860.21291294618822082No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGG4264930.21187318568187688No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCG4249080.21108578940736408No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGGGA4169750.20714483379492887No Hit
AAAACGATGCCGACCGGCGATGCGGCGGCGTTA3990590.19824452360302303No Hit
TCGACTGCATAATTTGTGGTAGTGGGG3716090.18460791304442645No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAT3703770.18399588010423734No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGAT3700720.18384436220914185No Hit
CTTTGGTGACTCTAGATAACCTCGGGCC3671980.18241661653535654No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3646750.18116324063592706No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3643530.18100327748110492No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAG3627750.1802193586664796No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAG3621170.1798924774370604No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCG3417520.16977555306453512No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC3372140.1675211654975074No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC3356640.1667511565224318No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATAC3303500.16411126768788237No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT3230490.16048427702528442No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTT3214910.1597102938103375No Hit
TCACCTCTAGCATCACCAGTATTAGAGGC3179760.15796411216685965No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATC3074200.152720102656603No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTA3073950.152707683157005No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTAG3017040.14988050826851784No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATA2922250.14517153080094272No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATT2875850.1428664716755552No Hit
TCGCCTGGATACCGCAGCTAGGAATAATGGAAT2798430.13902040104004865No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT2767930.13750522208909347No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGA2736930.1359652041389423No Hit
GGGGGGGGGATGCGTGCATTTATCAGAT2734960.1358673384821101No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2702770.13426820371387252No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTA2698380.13405011730093175No Hit
CGCCGCCTGGATACCGCAGCTAGGAATAATGGAAT2551500.12675341289711878No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGG2521820.12527896990484502No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCG2519940.1251855752678681No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG2477450.12307475711619319No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGC2475460.12297589789939316No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT2470090.12270912704802828No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATC2468750.12264255853018302No Hit
ACTTCGAACGCACTTGCGGCCCCGGGT2467780.1225943708717428No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA2432560.12084471176837754No Hit
CGGGCCGCCGGTGAAATACCACTACTCT2413160.11988095859957325No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTC2390110.11873588073663828No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATC2387300.11859628556115684No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCG2386450.11855405926252366No Hit
CCTCGACTGCATAATTTGTGGTAGTGGGGG2343060.11639853091229596No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2335960.11604581712371294No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2334870.11599166810546568No Hit
TATGCTAAGACTTCACCAGTCAAAGCGA2294550.11398865121030136No Hit
TGCCGCCTGGATACCGCAGCTAGGAAT2217210.11014655481466616No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGA2206240.10960158717230623No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGGCCGC2205230.10955141239393033No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGA2178220.10820960965736315No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG2168950.10774909461226956No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCG2149560.1067858382234492No Hit
TCTCGCCCGTCACGCACCGCACGTTCGTGGGGA2147840.106700392066215No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCGGCCG2111310.10488565478495625No Hit
TCTCGACTGCATAATTTGTGGTAGTGGGG2087930.10372418318255192No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCC2067410.10270479065554863No Hit
GACTGCATAATTTGTGGTAGTGGGGGA2065540.10261189279855563No Hit
AAAATCAGTTATGGTTCCTTTGGTCGCTC2027010.10069779952051293No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATAC2016780.10018959359696304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)