FastQCFastQC Report
Fri 14 Jul 2023
SRR9332878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9332878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42666157
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA11373082.6655974663947353No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA7890841.8494377171114802No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG7386601.7312550553826536TruSeq Adapter, Index 1 (100% over 49bp)
GGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT7371501.7277159506069413Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC5244801.229264683950795Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4992591.1701522590844073No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3574900.8378771962049453No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC3458510.810597964095993No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2290250.5367837557997079Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2074930.4863175279648458No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG1532230.35912069605893965No Hit
GGGTCGCGCCGTCGGGCCCGGGGAAGATCGGAAGAGCACACGTCTGAACT1486560.34841666194590715Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1256110.294404298001341Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1240560.2907597232157562Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA1185240.27779394333546376No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG1173160.27496265951489374No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA1133320.2656250479742059No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT1067780.25026392698081523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTCGCGCCGTCGGGCCCGGGGAAGATCGGAAGAGCACACGTCTGAAC1037290.2431177478674726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1032040.24188726441896324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT1000370.23446451950195563No Hit
GGGTCGCGCCGTCGGGCCCGGGGAGATCGGAAGAGCACACGTCTGAACTC991190.23231293130056216Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC988420.2316637047953487No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA910360.21336817374951297No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA742760.17408645451710122Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC740590.17357785469171738No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTC671030.15727453494346819No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA635400.14892365393958495No Hit
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC625070.14650253126851806Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC609250.14279467447700997No Hit
CGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCT607040.14227669953963748Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCCGGATGTGCTGACCCCTGCGAAGATCGGAAGAGCACACGTCTGAAC594060.139234475699323Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC583250.1367008516843924No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT565720.13259220885536985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG562970.13194766990615067No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCAC517990.12140535647492227No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT502950.11788031436719272No Hit
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC496580.11638732778300141Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGTCGGGGCGGCGGCGGCGGTAGATCGGAAGAGCACACGTCTGAACTC490780.11502793654464824Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT484500.11355604396243141Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG478470.11214274583014355No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT459340.1076590985215753No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA457270.10717393647616305No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTG456400.10697002779041008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCGCGCCGTCGGGCCCGGGGTAGATCGGAAGAGCACACGTCTGAACT445400.10439187199353342Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA444810.10425358909170095Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG444760.10424187020171514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT433290.10155355683897194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)