FastQCFastQC Report
Mon 17 Jul 2023
SRR9332879.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9332879.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44200400
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA7835241.7726626908353769No Hit
CGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC4705571.064598962905313Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA4685211.0599926697495952No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4580821.0363752364231997No Hit
GGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT4046850.915568637387897Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3442080.7787440837639479No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2799860.6334467561379534Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC2730380.617727441380621No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG2137430.4835770717007086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1944440.43991457090886055No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1526960.3454629369869956Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1318870.2983841775187555No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1274120.2882598347526267TruSeq Adapter, Index 2 (100% over 49bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA1233440.27905629813304855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA1118030.25294567469977647Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGTCGCGCCGTCGGGCCCGGGGAAGATCGGAAGAGCACACGTCTGAAC973020.22013827929159013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT961790.2175975783024588No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA895480.20259545162487216No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC884600.20013393543949828No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGA874600.19787151247500023Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT856040.19367245545289183Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCGCGCCGTCGGGCCCGGGGAAGATCGGAAGAGCACACGTCTGAACT846090.19142134460321628Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCT829410.1876476230984335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA824260.186482475271717Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG787210.17810019818825168No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT785820.17778572139618645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTC781880.17689432674817424No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA747860.16919756382295184No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG728390.1647926263110741No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG704380.15936054877331426No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT699870.15834019601632565No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC691290.15639903711278633Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG682740.15446466547814047No Hit
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA653300.1478040922706582Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG632690.1431412385408277Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC630720.14269554121682157Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC625900.1416050533479335No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTG615180.13917973592999158Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG604520.13676799304983667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT598310.13536302838888337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA589500.13336983375716058No Hit
CCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAAC587640.13294902308576392Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTC563820.12755993158432957No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC556090.12581107863277255No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA535680.12119347336223202No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT521890.1180735920941892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCAGATCGGAAGAGCACACGTCTGAA520730.11781115103030743Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC499950.11310983611008045Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT496050.11222749115392622No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAA480960.10881349490049863Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT480550.10872073555895422Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACAC453040.10249680998362005No Hit
ACTCCGGATGTGCTGACCCCTGCGAAGATCGGAAGAGCACACGTCTGAAC449470.10168912498529425Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTCT448360.10143799603623496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)