FastQCFastQC Report
Mon 17 Jul 2023
SRR9332882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9332882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35079021
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA9115962.59869281984808No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4724221.3467365580128363No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA4247241.2107635501002152No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3193400.9103446758106504No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2937130.83728961535158Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC2196330.626109263425567No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2011690.5734738150189539No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1801810.5136431829155096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1475190.4205334008608735Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1147410.32709293682967944No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1081680.308355241726957No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA962740.2744489363029829Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT923950.2633910450351508No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC823240.23468157791518754No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG778600.22195602323109304Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT724930.20665628040189604Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA712110.20300167441959113Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT643210.18336030529472302Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC640570.1826077187273841Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG633160.18049534506678508No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTC630680.1797883698065576No Hit
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA619970.17673526293678493Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT613070.17476827531760364Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA609120.17364224617328972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC602420.17173227268799776No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA515030.14681994688506272No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT505420.14408041775168126Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA494570.14098740098818607No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT494220.14088762625387977No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT488370.13921996283761737Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA474050.13513775085114263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAGATCGGAAGAGCACACG470080.13400602029343978No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG464280.13235261041064972No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT462980.1319820185403692No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAA459130.1308844964629999Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTG432670.1233415265494439Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC429810.12252622443482673Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA414700.11821880661948918Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCTG410520.11702721122120255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGAGATCGGAAGAGCACACGTCT409820.11682766175258996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT407300.11610928366558462Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG403350.1149832545212707TruSeq Adapter, Index 5 (100% over 49bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTCT389050.11090674394818488Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACA373280.1064111794910126No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG363320.10357187562332483No Hit
CCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAAC362500.10333811767437866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)