FastQCFastQC Report
Tue 18 Jul 2023
SRR9332883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9332883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37797813
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA10160632.688152883342748No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC7847202.076098953132553No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA4337631.1475875601585732No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT3757990.9942347722605009No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC3325910.8799212801015763No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCT2988070.7905404474063089Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAACT2524640.6679328245790306Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACACGTCTGAAC2320550.6139376370796903Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2024230.535541566915525No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC1868600.49436722701390157No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1843620.48775837903637437No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTG1689100.4468777069191808Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1590220.42071746320349274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1357720.35920596781618025No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTG1164750.30815274947256865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA1077540.28507998597696643Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT1033880.2735290531227296Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG956790.2531336932112977TruSeq Adapter, Index 6 (100% over 49bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGA942590.24937686209516938Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCT910240.24081816585525728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT848130.2243859982057692No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC841240.22256314141773226Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT803720.21263664117286363Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTC794430.2101788270130867No Hit
GCCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAA790180.20905442333396382Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT772420.20435573878308777No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCT761520.20147197405310197Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCACA758780.2007470643870321No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG734510.19432605796531138Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC726930.1923206509328992No Hit
CCCTGTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGA637830.16874785850705173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCTGTGGTCTAGTGGTTAGGATTCGAGATCGGAAGAGCACACGTCTGAA611430.16176332741791172Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG584700.15469148969015747No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA562400.14879167744440663No Hit
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC554130.14660372016761922Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTGATCGTATAGTGGTTAGTACTAGATCGGAAGAGCACACGTCTGAAC509350.13475647387323705Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCTGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTCTG506480.13399717068286462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT498340.1318436069303798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGAAGATCGGAAGAGCACACGTCT492610.13032764620535056Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT477830.1264173670577184No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGTC473370.12523740460856822No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC472060.1248908237098268No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTCT443260.11727133524894681Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGAGATCGGAAGAGCACACGT435260.11515481067648015No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG430400.11386902199870665No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA420940.1113662316917648Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCAC415610.10995609719535888No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGTAGATCGGAAGAGCACACGTCT394740.10443461371693649Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAG393000.10397426962242498No Hit
GGGTCGCGCCGTCGGGCCCGGGGAAGATCGGAAGAGCACACGTCTGAACT390990.10344249282359273Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCAAGATCGGAAGAGCACACGTCTG387790.10259588299460606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)