FastQCFastQC Report
Fri 29 Sept 2023
SRR9336389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9336389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138051092
Total Bases7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTAAAAAGCTCGTAGTCGAACTTCAGCTGTAGGCACCATCAATATCTC14174571.0267626133663614No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA10247930.7423287894021149Illumina PCR Primer Index 6 (96% over 29bp)
AAAATCAGTTATAGTTTATTTGATGGTATCTGTAGGCACCATCAATATCT4548930.3295106133604506No Hit
TAGGGAAACTCCGTTTTAAAGCGCACGACTGTAGGCACCATCAATATCTC3998540.28964204064390886No Hit
ATCAGTTCTGATCGGTGGATAAGGGCTGCTGTAGGCACCATCAATATCTC3489660.2527803257072389No Hit
TGGCCGAGAAGTTGATCAAACTTGGTCATTTCTGTAGGCACCATCAATAT3408350.24689047733139263No Hit
AAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTC3301940.23918246151939168No Hit
ATCGAATCTTTGAACGCAACTTGCGCCCTTCTGTAGGCACCATCAATATC3187730.23090943750013943No Hit
AAATGAAAGTGAATGTAGGAGGGATCCGCCTGTAGGCACCATCAATATCT3032690.21967881282677573No Hit
TTAACCTATTCTCAAACTTTAAATGTGTCTGTAGGCACCATCAATATCTC2551990.18485837113117512No Hit
ATCTGTAAAAAGATAACGCAGGTGTCCTCTGTAGGCACCATCAATATCTC2311240.16741917550351576No Hit
TTGCAGTTAAAAAGCTCGTAGTCGAACTTCTGTAGGCACCATCAATATCT2173740.15745909492697097No Hit
ATGAGGTAAAGCGAATGATTAGAGGCCTGTAGGCACCATCAATATCTCGT1998060.14473337161288083No Hit
AAAAAAGCTCGTAGTCGAACTTCAGCTGTAGGCACCATCAATATCTCGTA1980680.14347441742800557No Hit
TTTGCAGTTAAAAAGCTCGTAGTCGAACTTCTGTAGGCACCATCAATATC1825020.13219888184586037No Hit
CTCTGACTGGGTCGGCACCTTACGAGAAATCCTGTAGGCACCATCAATAT1595450.11556953131526117No Hit
AGAAGCCTAGGCAGTGATGTCGGGTGGAACCTGTAGGCACCATCAATATC1413670.10240194260832068No Hit
TTTGGATTTGGGTGTTTGCCGCGACCTCTGTAGGCACCATCAATATCTCG1405090.1017804335803443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)