FastQCFastQC Report
Fri 29 Sept 2023
SRR9336391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9336391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172330085
Total Bases8.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTAAAAAGCTCGTAGTCGAACTTCAGCTGTAGGCACCATCAATATCTC11921810.6918008541572994No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA9531510.5530961120340653Illumina PCR Primer Index 6 (96% over 29bp)
TTGGAATGAGTACAATTTAAATCCCTTAACGACTGTAGGCACCATCAATA4782490.27751915749359723No Hit
AAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTC3810760.22113144086245878No Hit
TGGCCGAGAAGTTGATCAAACTTGGTCATTTCTGTAGGCACCATCAATAT3282290.190465292232636No Hit
AAAATCAGTTATAGTTTATTTGATGGTATCTGTAGGCACCATCAATATCT3137090.18203960150080586No Hit
CTCTGACTGGGTCGGCACCTTACGAGAAATCCTGTAGGCACCATCAATAT3136030.18197809163733655No Hit
ATCGAATCTTTGAACGCAACTTGCGCCCTTCTGTAGGCACCATCAATATC2825220.16394235516102718No Hit
ATCAGTTCTGATCGGTGGATAAGGGCTGCTGTAGGCACCATCAATATCTC2438020.14147384654281348No Hit
AGCCCCGACTTCTGGAAGGGGTGTATTTATTAGACTGTAGGCACCATCAA2355310.13667433634701684No Hit
TAGCCCCGACTTCTGGAAGGGGTGTATTTATTAGACTGTAGGCACCATCA2340230.13579927149690665No Hit
AGCCCCGACTTCTGGAAGGGGTGTATTTATTAGATCTGTAGGCACCATCA1919990.11141351203998999No Hit
TAGGGAAACTCCGTTTTAAAGCGCACGACTGTAGGCACCATCAATATCTC1861720.10803221039437194No Hit
TTGCAGTTAAAAAGCTCGTAGTCGAACTTCTGTAGGCACCATCAATATCT1791460.10395515095347396No Hit
TTTGCAGTTAAAAAGCTCGTAGTCGAACTTCTGTAGGCACCATCAATATC1785520.10361046360535364No Hit
TAGCCCCGACTTCTGGAAGGGGTGTATTTATTAGATCTGTAGGCACCATC1778560.1032065875206874No Hit
TGGAATGAGTACAATTTAAATCCCTTAACGACTGTAGGCACCATCAATAT1734610.10065624931363552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)