FastQCFastQC Report
Fri 29 Sept 2023
SRR9336393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9336393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160189934
Total Bases8.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCGCATACACTGGTTGGGACTGAGGACTGTAGGCACCATCAATATCT35887602.2403155494152336No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA16455901.027274285536568Illumina PCR Primer Index 6 (96% over 29bp)
AAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTC13795770.8612132894692373No Hit
TTTCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATA13752530.8585139937694213No Hit
CCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCT11255040.7026059452649503No Hit
TCGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATC9977260.6228393851513792No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCT6786300.4236408512410025No Hit
CGTTTTTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATATCTC6175330.38550050217262716No Hit
AGTTAAAAAGCTCGTAGTCGAACTTCAGCTGTAGGCACCATCAATATCTC5979750.373291245628455No Hit
CGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCTC5633610.3516831463330274No Hit
TCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATC5476000.34184420102201923No Hit
TTGTCGCATACACTGGTTGGGACTGAGGAACTGTAGGCACCATCAATATC5149000.3214309333568987No Hit
ATTACCCAATCCCGACACGGGGAGGTAGTGCTGTAGGCACCATCAATATC4033690.2518067084040374No Hit
AAGGAGCACCTTCGACCGATCCGGCTGTAGGCACCATCAATATCTCGTAT3983940.24870102012776907No Hit
TCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCT3912880.24426503602904287No Hit
ATGGTGAATCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAATA3707980.2314739701434673No Hit
ATGGTGAATCATAATAACTTCTCGAATCGCACTGTAGGCACCATCAATAT3216510.2007935155276361No Hit
TTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGT3130850.19544611336190448No Hit
TTCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATAT3110120.19415202455854685No Hit
AAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTCGT2862540.17869662147435558No Hit
ATTCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATA2554650.15947631266269202No Hit
TCGTTTTTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATATCT2231110.13927903859427274No Hit
TATGGTGAATCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT2179430.13605286834065367No Hit
AAAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCT2155920.134585235549195No Hit
TTTCCGAAGGGCATGCCTGTTTGAGAGTCATCTGTAGGCACCATCAATAT2000570.12488737276088772No Hit
TTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGTA1983900.12384673309123156No Hit
ATGTCGCATACACTGGTTGGGACTGAGGACTGTAGGCACCATCAATATCT1909030.11917290633255395No Hit
TATGGTGAATCATAATAACTTCTCGAATCGCACTGTAGGCACCATCAATA1828430.11414137919552422No Hit
ATTCCGAAGGGCATGCCTGTTTGAGAGTCATCTGTAGGCACCATCAATAT1744430.10889760401549325No Hit
AAATGAAAGTGAATGTAGGAGGGATCCGCCTGTAGGCACCATCAATATCT1734050.10824962322538943No Hit
ATCGAATCTTTGAACGCAACTTGCGCCCTTCTGTAGGCACCATCAATATC1673520.1044709838010171No Hit
TTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCG1631430.10184347788045157No Hit
TTTCCGAAGGGCATGCCTGTTTGAGAGTCCTGCTGTAGGCACCATCAATA1608250.10039644563434305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)