FastQCFastQC Report
Fri 29 Sept 2023
SRR9336395.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9336395.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169471324
Total Bases8.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTCGCATACACTGGTTGGGACTGAGGACTGTAGGCACCATCAATATCT50755012.9949025476428095No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA17016871.0041150088613222Illumina PCR Primer Index 6 (96% over 29bp)
AAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTC12458450.7351361697038491No Hit
CCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCT11001700.6491776744483332No Hit
TTTCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATA10204390.6021307770039018No Hit
TCGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATC8816190.5202172138573721No Hit
CGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCTC6986400.4122467350287533No Hit
TTGTCGCATACACTGGTTGGGACTGAGGAACTGTAGGCACCATCAATATC6428440.3793231709218251No Hit
CGTTTTTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATATCTC6373080.3760565415775002No Hit
AAGGAGCACCTTCGACCGATCCGGCTGTAGGCACCATCAATATCTCGTAT5724060.3377597970497947No Hit
CGTTTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCT4528350.2672044976765509No Hit
TCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATC4442250.26212399213922466No Hit
TCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATATCT4114200.24276673497871534No Hit
AGTTAAAAAGCTCGTAGTCGAACTTCAGCTGTAGGCACCATCAATATCTC3798680.22414883594111767No Hit
AAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCTCGT3790880.2236885810840777No Hit
TTTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGT3542760.20904775606756928No Hit
ATTACCCAATCCCGACACGGGGAGGTAGTGCTGTAGGCACCATCAATATC3106540.18330770815244235No Hit
ATGGTGAATCATAATAACTTCTCGAATCGCACTGTAGGCACCATCAATAT2899590.1710962026826438No Hit
TTTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATATCTCGTA2774450.16371206257879947No Hit
TCGTTTTTTTACTTATTCAATGAAGCGGACTGTAGGCACCATCAATATCT2571600.15174248594411172No Hit
ATGTCGCATACACTGGTTGGGACTGAGGACTGTAGGCACCATCAATATCT2474560.14601644346627043No Hit
TTCCGAAGGGCATGCCTGTTTGAGAGTCATGCTGTAGGCACCATCAATAT2439990.14397657033705596No Hit
TACCTGGTTGATCCTGCCAGTAGTCTGTAGGCACCATCAATATCTCGTAT2231600.13168009473980388No Hit
TTTCCGAAGGGCATGCCTGTTTGAGAGTCATCTGTAGGCACCATCAATAT2008260.11850146399989181No Hit
ATGGTGAATCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAATA1835500.10830740898678529No Hit
AAAAAGGACGTCGGCTCGCCTTAAATGTGCTGTAGGCACCATCAATATCT1776310.1048147827062471No Hit
TATGGTGAATCATAATAACTTCTCGAATCGCACTGTAGGCACCATCAATA1755830.10360631867135234No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)