FastQCFastQC Report
Mon 25 Sept 2023
SRR942870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66618455
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCAC30705954.609225776851175No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA24671083.7033401630223937No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA20243143.0386684890845936No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA18880812.834171101686462No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT11401761.7115017152529282No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACC9117001.3685396936929863No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCA8367261.2559973058516594No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACCA7863861.1804326593884533No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTC6030430.9052191318456726No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA5538110.8313176881691418No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT5156410.7740212528195077No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA4471990.6712839557747174No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTCG4247290.6375545635214747No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTC3903790.5859922749634466No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGTA3468520.5206545243356364No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT3354880.5035961881733823No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATT2854870.42854040971079266No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2638030.39599087069791694No Hit
AGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGCTGTAGGCACCATC2503800.3758417993932762No Hit
TAACCAAACGTAATGTTACGGTGCCCAAATTAACAACCTGTAGGCACCAT2360970.35440179451775033No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT2235000.3354926198753784No Hit
AACTCCATCTAAAACTAAATATAACCATGAGACCGCTGTAGGCACCATCA1998500.29999194667603746No Hit
TTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACC1906460.28617595529647155No Hit
AACTCCATCTAAAACTAAATATAACCATGAGACCGACTGTAGGCACCATC1906440.28617295312537644No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT1897440.2848219761325897No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA1762180.2645182930165523No Hit
TGGGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCAC1721730.2584464019767495No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT1688800.2535033272686975No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA1688190.25341176105029756No Hit
AAGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGCTGTAGGCACCAT1645780.24704565724317684No Hit
TGGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA1634390.24533592080452782No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCCTGTAGGCACCATCA1587250.23825980353342027No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT1559970.23416484215972888No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA1552150.23299099326155193No Hit
CGCTAGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCA1519180.22804191421130973No Hit
TGAACCAAACGTAATGTTACGGTGCCCAAATTAACAACCTGTAGGCACCA1513510.2271907987058541No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA1428790.2144736019470881No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT1417680.2128058959037702No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATCA1397850.20982924326299673No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC1388800.20847076084247224No Hit
TTTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCAC1383690.20770370612767886No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACCATC1377780.20681656456908223No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCACTGTAGGCACCAT1353240.20313290063541703No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATT1319610.19808474993903716No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT1317580.19778002957288637No Hit
TAACCAAACGTAATGTTACGGTGCCCAAATTAACAACTCCTGTAGGCACC1296420.19460373255428995No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA1275980.19153551369511648No Hit
TAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGCTGTAGGCACC1258340.18888759878925443No Hit
TCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTCGTA1248200.187365498044048No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTCGT1220260.1831714650242189No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCA1211110.18179797174821902No Hit
ACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGCTGTAGGCACCAT1192040.17893540160905863No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATT1188010.17833046413339967No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATTC1173100.17609234558201628No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC1067610.16025739414100793No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1035610.15545392038887723No Hit
CTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA973290.1460991552566027No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCG958000.14380399545441275No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATT956880.14363587387308818No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTC939420.14101497850708186No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC919870.13808035626163953No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC915720.13745740575941007No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATT913030.13705361374712158No Hit
ACCAAACGTAATGTTACGGTGCCCAAATTAACAACCTGTAGGCACCATCA910920.13673688469659045No Hit
GTGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCA892990.13404543830984972No Hit
ATAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA877200.13167522423028274No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC861760.12935754814487968No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCACTGTAGGCACCATC854690.1282962806627683No Hit
ACTGCGATCCACTGAAGCTTATCCTTTGCTTGATGATTCGACTGTAGGCA833630.1251349944996473No Hit
TGGAACGTACCTTGAGCATATATGCTGTGACCCGACTGTAGGCACCATCA833080.12505243479453254No Hit
CGCTAGTTACATATAAAAGCATTATTTAATACAATGCTGTAGGCACCATC821360.12329316253281468No Hit
ACCGAAAGGTTGGTGGTTCGTGTCCACCCGGGGGCGCCACTGTAGGCACC783190.11756351899785127No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATT769710.11554005567976622No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGT748710.11238777602993046No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC671210.10075436303648891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)