FastQCFastQC Report
Mon 25 Sept 2023
SRR942874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27730622
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC18179376.555702212521594No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA7668022.7651813940560004No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT4907111.7695636253669318No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC4701311.6953496391101504No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT4696961.693780976135335No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA4567351.6470420317294No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC4168071.5030568012502568No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT3339781.2043653402365082No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG3011351.0859294825770587No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA2708940.9768767537922518No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA2433210.8774451579196457No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA2400820.8657649294703884No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA2174760.7842449404849267No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT2111200.7613244304437167No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA1765960.6368266820700957No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT1693280.6106173889644452No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC1586960.5722771021868893No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA1531240.552183791622128No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT1461380.5269914248587717No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT1245650.4491965596732738No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC1207210.4353346275463998No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC1115830.40238188670993386No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT1113930.40169672357150876No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT928460.3348139828958759No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATAT847740.3057053678781529No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA820330.2958209880759256Illumina PCR Primer Index 6 (96% over 29bp)
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT794760.2866001346814363No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAT773240.27883976060832677No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT749120.2701417948721092No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT730960.2635930777174778No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA683620.2465216972053494No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT662290.23882983944608238No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC637220.22978929214065233No Hit
AACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCAATATCTCG615760.2220505547982299No Hit
AGCATTGTGATGGCCCTAGCGGGTGTTGACACAATGCTGTAGGCACCATC588650.21227435864943817No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAA580420.20930652042352316No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA557370.20099440971789237No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC555440.20029842821412372No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATATCT513170.18505535144505594No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAAT497070.17924949537734855No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC493840.17808471804202589No Hit
ATATGGGACTACCCCCTGAATTCTGTAGGCACCATCAATATCTCGTATGC484200.17460841664496382No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA481590.17366721886007463No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATAT474980.17128357236271152No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATA474930.1712655417538056No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT471900.17017288685410664No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC465730.16794790971511564No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATCTC463540.16715816904503622No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT444840.1604147213142208No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT439240.15839529311675737No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC420440.1516157841681301No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA412290.14867679491646454No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT401320.1447208793225049No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATATCT394430.14223626141526866No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT377480.13612388499616054No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC372910.13447588734215915No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTCCTGTAGGCACCATCAAT372080.1341765792343208No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA364810.13155492869939953No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAAT363690.13115104305990685No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATATCTCGTATG359610.12967974537318347No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATA349490.12603035013062455No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA344490.12422728924003218No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATATCT343220.12376931177382174No Hit
TGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG341020.1229759649819611No Hit
AACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAATATCTCGTA333990.12044086136978824No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA332950.12006582470454503No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT332380.1198602757630175No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA331880.11967996967395826No Hit
GATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCGT321980.1161099091105854No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAATA321280.11585748058590248No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAT311240.11223693431759302No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA303470.10943497769361249No Hit
AACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATATCTCGT300840.10848656766516093No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAAT299510.10800695346826336No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT294650.10625437828260759No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATATCT293520.10584688652133371No Hit
ACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAATATCT279290.10071537522670787No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)