FastQCFastQC Report
Mon 25 Sept 2023
SRR942875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28089260
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC22745348.09752197103092No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA7580192.69860793769576No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC5278151.879063385792292No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG5239151.8651790755612643No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA5031431.7912291032230823No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT4680661.6663521929733998No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT4315171.5362348456313908No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT4208951.4984196806893453No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA3893861.3862451342612798No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC3449051.2278892359570883No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA3177061.1310586323740819No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT3174011.1299728081124245No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA2310960.822720142858872No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA2253590.8022959665010755No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT2102360.7484568835206054No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC1939430.6904525074708269No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA1766950.6290482554542198No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT1665580.5929597290921869No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA1463280.5209393198681631No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC1389710.4947478146451704No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT1345750.4790977049591196No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT1170580.4167357915445263No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT1119320.39848682378959077No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC1016020.36171120207509916No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATA1000410.3561539179031416No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT943050.3357333016248915No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA941800.33528829168158936Illumina PCR Primer Index 6 (96% over 29bp)
ATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATAT712500.2536556676822387No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA684610.2437266058272806No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC654730.23308908814258544No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT610310.21727521479739942No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT605630.2156090975696761No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA589590.20989872997722261No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAT580270.20658073583996162No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT577270.20551271197603638No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT541960.1929420710976366No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGCTGTAGGCACCATCAATA532360.18952439473307592No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC526450.18742038772114325No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA522490.18601059622076194No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT517660.18429107779984236No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA500540.1781962216163758No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC500450.17816418090045805No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATATCT490650.174675302944969No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAATAT474770.16902189662525818No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAA473490.16856620644331677No Hit
TTAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATAT456770.1626137534417069No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC454690.1618732568960521No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT430200.1531546220868759No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA420220.14960166269955136No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC405040.14419746194808977No Hit
TAGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAAT396970.14132447775413096No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATA392820.13984704474236773No Hit
AGGACACTAGCTTCTTAAATGGACAAATTGCGCTGTAGGCACCATCAATA385240.13714850444618334No Hit
TCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATATCTC382650.13622644384366125No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT369400.1315093384446582No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCGTCCTGTAGGCACCATCAAT363970.12957621525095356No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATATCT361580.12872535623935982No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT357710.12734760545489626No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT355850.12668543065926266No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATATCTCGTATG355400.12652522707967387No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATATCT346620.1233994772379194No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA325910.11602655249728899No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT325470.11586990899724664No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT323830.11528605595163419No Hit
TTATGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATATCTCG322700.114883766962889No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC315990.11249495358724296No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA314470.11195382149618752No Hit
AACTCCATCTAAAACTAAATATAACCATGAGACCGCTGTAGGCACCATCA307800.10957924843872711No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA304770.10850054433616264No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA303870.10818013717698509No Hit
TACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT297300.10584116491498885No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAAT295880.1053356336193976No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC289550.10308210326651539No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA284920.10143378643652415No Hit
GATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCGT281640.10026608034529924No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)