FastQCFastQC Report
Mon 25 Sept 2023
SRR942876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32237241
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC26207838.12967524112873No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA5896621.8291329583694833No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT5044121.5646872509964487No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC4993431.5489632006659628No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG4951961.536099196578268No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC4674811.4501271991607472No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT4110521.2750843038956094No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA3652531.1330156944882475No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT2883510.8944655034219585No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC2392020.7420051858656266No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC2364710.733533617222392No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT2212870.6864328122868827No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT1967200.6102259185269608No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1939030.6014875776745287Illumina PCR Primer Index 6 (96% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA1845850.5725831190082303No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA1797870.5576997113369596No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1689680.5241391470194363No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA1672650.5188564368768407No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA1373900.4261841142050587No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT1226320.3804047623058065No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT1200920.3725256761271847No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC968770.30051268965604094No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT855390.2653421860760355No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCA840100.26059922435669974No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA803080.24911561135148014No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC771370.23927916163793295No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT709140.21997540050030956No Hit
GCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATATCTCGTATG693770.21520762276151362No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA684230.21224831244088166No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT636120.19732457873798814No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC603740.18728029486146164No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT593170.18400147829027924No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT582890.1808126197896402No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC575440.17850162797740662No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA549610.17048915569418613No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA532900.16530570962943136No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT516620.16025564966927536No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT507430.15740490943378188No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC498100.154510741164233No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT497090.15419743891854765No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC493390.1530496980185122No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT488190.1514366567535975No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA461570.14317912627820725No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAT428040.13277811212193996No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT410720.127405443908801No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC405830.1258885647192947No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATATC400310.12417625937653907No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC389760.12090364681022175No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA387090.12007541216073671No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT382330.11859885900285326No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATA379950.11786058242391152No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAATA337680.10474841814161454No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATA324040.10051728682364598No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA322870.10015435253904016No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)