FastQCFastQC Report
Tue 26 Sept 2023
SRR942877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40195868
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC29090137.23709461878022No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA8299012.064642564753173No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC6368451.584354391849431No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTCG6007051.4944446528682997No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATATC5407501.3452875305491598No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCA5169771.2861446355630384No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT5024551.2500165439890487No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCT4658971.1590668971248488No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT3935260.9790210277334973No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT2866700.7131827579889555No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCTC2786710.6932827025902265No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAA2559170.6366748940463234No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCT2467650.6139063846064974No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA2459750.611941008463855No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1966840.48931397625248446No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATA1834520.4563951697721766No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA1543690.38404196172601623No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATCTC1539060.3828901020373537No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATATCT1488420.3702917921812262No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCATCAATAT1276970.3176868826417681No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT1248960.3107185046980451No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA1208830.3007348914570025No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATATC972690.24198755951731157No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAAT861020.21420609700479662No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAT826220.20554849070556208No Hit
AGTTACATATAAAAGCATTATTTAATACAATGACCTGTAGGCACCATCAA805320.20034895129021718No Hit
AGCAGAGTGGCGCAGTGGAAGCGTGCTGGGCCCTGTAGGCACCATCAATA761280.18939260124946175No Hit
AAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC759340.18890996457645845No Hit
CTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA738170.18364325407775745No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATAT716010.17813024960675064No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA675110.16795507438724797Illumina PCR Primer Index 6 (96% over 29bp)
CGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATCTC673080.16745004735312596No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAT666620.16584291698838297No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATAT663800.16514135233999674No Hit
CAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATATC636550.15836204855683175No Hit
TGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGATCGCTGTAGGCACCA633190.15752614174173327No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC616680.153418754385401No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCTGTAGGCACCATCAATA604100.15028907946458575No Hit
GCGCAGTGGAAGCGTGCTGGGCCCCTGTAGGCACCATCAATATCTCGTAT556340.13840726116425697No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC554890.13804652756845553No Hit
TTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAT538670.13401128693128359No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATATC533560.13274001198332128No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA520860.12958048324768107No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATAT517240.12867989316712852No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATAT507830.12633885652127227No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCTGTAGGCACCATCAATA485500.12078355914593013No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATATC471630.1173329557157467No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATATCT468630.11658661034512305No Hit
GTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA456420.11354898468668471No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATA451100.11222546556277874No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATATC446300.11103131296978089No Hit
TGTTACGGTTGTTTCGCAAAAGTTGACCGAACTCTGTAGGCACCATCAAT444750.11064570119495865No Hit
TTAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATAT441630.10986950200951003No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTTGACTGTAGGCACCATCAAT432960.10771256388840762No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATA428510.10660548492198252No Hit
AGTGAGGTCTCCGGACGTGATCACTGTGACGCCCTGTAGGCACCATCAAT402260.10007496292902544No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)