FastQCFastQC Report
Mon 25 Sept 2023
SRR942880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR942880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79046292
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTCG23196182.934505770365547No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA15047051.90357442699526No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATTCG13855811.7528728608800523No Hit
TGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC7206040.911622774158717No Hit
CGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT5314290.6723009853517228No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA4876390.6169030673823384No Hit
TTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCGTAT4505410.5699710746710295No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT4481080.5668931314324017No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTCG4402880.5570001942659119No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT4289230.5426225432560454No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA3964970.5016010112150485No Hit
AATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCAA3924450.4964749010617727No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA3843750.48626569352551036No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCG3690500.4668783198584445No Hit
TTGTATGGCTTCTAAACCATTTAAAGTTTACTGTAGGCACCATCAATTCG3215050.4067300209350744No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC3163660.4002287672140269No Hit
AGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTC3158520.39957851533377425No Hit
TCGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTA2945290.37260318295512207No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTC2738880.3464906361452097No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAAT2681650.3392505748403733No Hit
AAATTAACGATTTAAGTCCTTCTTAAATGAGGCCACTGTAGGCACCATCA2563140.3242580942316687No Hit
TGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGTA2543790.3218101615696281No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2522300.3190915014710621No Hit
TTGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTAT2271640.2873809691161731No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTCG2165500.2739533942971038No Hit
ATGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTCGT2050690.25942899383566276No Hit
TTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAATT1989870.25173476827983277No Hit
TCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAATTCGTA1966510.24877953794467675No Hit
TTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTCGTATG1957190.2476004820061642No Hit
TTGAATTATTTAGTGAGGTCTCCGGACGTGCTGTAGGCACCATCAATTCG1855140.234690325512043No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC1831190.23166045536962063No Hit
ACCGCTTGGGAACACCGCGTGTTGTTGGCCTCTGTAGGCACCATCAATTC1827260.23116327834833794No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATT1780850.22529203520387772No Hit
ATGTATGTAAGCGTATTACCGGTGGCTGTAGGCACCATCAATTCGTATGC1751670.22160052744789094No Hit
TAAGGTCGTATCCGTGCTGGACTGCAATGATAACTGTAGGCACCATCAAT1676190.21205169244371386No Hit
AGTTACATATAAAAGCATTATTTAATACAATGCTGTAGGCACCATCAATT1633250.20661943257249815No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGCTGTAGGCACCATCAAT1626150.20572122472234372No Hit
TGTATGGCTTCTAAACCATTTAAAGTTTACTGTAGGCACCATCAATTCGT1610750.20377299924454395No Hit
TGCACGCGCGCTACAATGAAAGTATCAACGCTGTAGGCACCATCAATTCG1508460.19083248079492457No Hit
CGCTAGTTACATATAAAAGCATTATTTACTGTAGGCACCATCAATTCGTA1462870.18506497433174982No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATT1330130.1682722827782991No Hit
ACGCGCGCTACAATGAAAGTATCAACGCTGTAGGCACCATCAATTCGTAT1326010.16775106920891367No Hit
AGGGAATACGGTTCCAATTCCGTAACCTGTTGAGCTGTAGGCACCATCAA1311020.16585471207175664No Hit
TACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAA1302020.16471613874057495No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCATTGGCTGTAGGCACCATCAATTC1279480.1618646450867044No Hit
TACACACAGGGACAGCAAAAGCTCGGCCTATCGCTGTAGGCACCATCAAT1204550.15238538956387734No Hit
TTTGCGGTTAAAACGTTCGTAGTTGAACTTGTGCTGTAGGCACCATCAAT1194160.15107096990710203No Hit
CGCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATTC1190090.1505560817451121No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCGCTGTAGGCACCATCAATTCGT1155320.14615739344231354No Hit
AGTTCGTGGAGTGATTTGTCTGGTTCTGTAGGCACCATCAATTCGTATGC1131400.14313131854432842No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1120640.14177009087282677Illumina Single End Adapter 1 (95% over 22bp)
TGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTATG1093470.13833286449413718No Hit
TTGGCAAATGTGAGATGCAGTGTATGGAGCTGTAGGCACCATCAATTCGT1092670.1382316579758099No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA1034340.1308524377082735No Hit
GGCTCGTTGGTCTAGGGGTATGATTTCCGCTTCTGTAGGCACCATCAATT1029500.1302401382723936No Hit
TATGCACTTGTATGATTAACAATGCGCTGTAGGCACCATCAATTCGTATG1014100.12829191279459384No Hit
TGATGCCAGCTAGCAATTGGGTGTAGCCTGTAGGCACCATCAATTCGTAT1010220.1278010611807066No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGACTGTAGGCACCATCAATT1003750.1269825534637349No Hit
CGCTAGTTACATATAAAAGCATTATTTCTGTAGGCACCATCAATTCGTAT965160.12210060403592365No Hit
AGCTATAAAATAAATTTTTAAAAATATTCTGTAGGCACCATCAATTCGTA949710.12014605315072843No Hit
ATTTAGAAATTATAAATTTACTTAATTATTCTGTAGGCACCATCAATTCG916800.11598267000304074No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATTGGACCTGTAGGCACCATCAATT895330.11326654006743289No Hit
TTCGCAAAAGTTGACCGAACTTGATTATTTAGCTGTAGGCACCATCAATT892140.11286297907560294No Hit
ATCAGTTATGGTTCCTTAGATCGTTCTGTAGGCACCATCAATTCGTATGC885580.11203308562531941No Hit
TCGCAAAAGTTGACCGAACTTGATTATTTAGCTGTAGGCACCATCAATTC862800.10915123001595066No Hit
ATGTGCTTTTATTAGGCTAAAACCAAGCTGTAGGCACCATCAATTCGTAT858640.10862495612064889No Hit
TATCCACTGAAGCTTATCCTTTGCTTGCTGTAGGCACCATCAATTCGTAT838880.10612515511796555No Hit
ATTGAATTATTTAGTGAGGTCTCCGGACGTGCTGTAGGCACCATCAATTC825300.10440717446936032No Hit
GCCCGGGTAGCTCAGTCGGTAGAGCATTGGACTGTAGGCACCATCAATTC816080.10324076934563862No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGACTGTAGGCACCATCAATTCG802400.10151013788224246No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)