FastQCFastQC Report
Thu 29 Jun 2023
SRR944653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR944653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29565498
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC15415625.214057277168137No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT8440612.854885109663974RNA PCR Primer, Index 1 (100% over 41bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG5598381.8935517338486907No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGC3412591.154247427186919No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT3386231.1453316294553875No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC3144201.063469318189736No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT2837550.9597504496626439No Hit
ACTGGGGCGGTACACCTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1854310.6271871354915112RNA PCR Primer, Index 1 (100% over 30bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCC1610160.5446077722079973No Hit
ACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA1569580.5308823142434469No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTG1320540.44664899606967556No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA1138190.3849723755710118No Hit
TCGAGGCCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA1094520.3702017804672189RNA PCR Primer, Index 1 (100% over 37bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCA1045770.35371296637722793No Hit
ACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC847820.28675992536976713No Hit
ACGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC824180.27876411890643615RNA PCR Primer, Index 1 (100% over 42bp)
AGACGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA740980.2506232095261849RNA PCR Primer, Index 1 (100% over 40bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAG722320.24431179884066215No Hit
AACTCCATCTAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA695810.23534526629654606RNA PCR Primer, Index 1 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGGGC672150.2273426951915371No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAGG645320.21826792838057388Illumina Small RNA Adapter 2 (100% over 21bp)
TTCGAGGCCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC643750.2177369040088552RNA PCR Primer, Index 1 (100% over 36bp)
AACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGT600690.20317263047623957No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACATGGAATTCTCGGGTG596960.20191102480330284No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGTGGAATTCTCGGGT538710.18220900591628797No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC517880.17516363160870824No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC507150.17153440134849074No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATT494350.1672050306746059No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA467200.15802202959679557No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGT465120.1573185068622893No Hit
ACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGTGG455840.1541797131237228No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC441360.1492821125488906RNA PCR Primer, Index 1 (100% over 50bp)
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGTGGAA431060.1457983220847489No Hit
ATGTGCTTGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA412750.13960529262859026RNA PCR Primer, Index 1 (100% over 37bp)
AGTGGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGTG402390.13610120823941474No Hit
TGGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT401340.13574606455132263RNA PCR Primer, Index 1 (100% over 43bp)
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGTG386150.1306083191969234No Hit
ATGTGTTGTTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA376470.127334232624798RNA PCR Primer, Index 1 (100% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA370780.12540969206742264No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGG365500.12362382666444514No Hit
TCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC358030.1210972329977327RNA PCR Primer, Index 1 (100% over 42bp)
CGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT353020.11940269025740746RNA PCR Primer, Index 1 (100% over 43bp)
ACCAAGCGTTGGATTGTTCACCCACTAATAGGGTGGAATTCTCGGGTGCC345850.11697756621586418No Hit
ATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGGAACTCC333860.11292216352993614RNA PCR Primer, Index 1 (100% over 27bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC327680.11083188925145114No Hit
AGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG326540.11044630467580827RNA PCR Primer, Index 1 (100% over 39bp)
ACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAAC320100.10826809005550998RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGGGTCGGGCCTGGTTAGTACTTGGATGGGTGGAATTCTCGGGTGCC317470.10737853967486022No Hit
TCGAGGCCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC314670.1064314898399479RNA PCR Primer, Index 1 (100% over 36bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT298680.10102315881843087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)