FastQCFastQC Report
Thu 29 Jun 2023
SRR944655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR944655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26371551
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC11755234.457542144563283No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT6709142.54408244702786RNA PCR Primer, Index 3 (100% over 41bp)
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGC4891541.854854877515547No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG3434811.3024679511644954No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT3405551.2913726613956078No Hit
ACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGTGGAA2351220.8915744091047204No Hit
AGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC1812840.6874225941432114RNA PCR Primer, Index 3 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1800930.6829063637553968No Hit
ACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAA1693930.642332337601228No Hit
ACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGTGG1635550.6201948455743085No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1505550.5708992997795237No Hit
ACTGGGGCGGTACACCTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1439320.5457851151796115RNA PCR Primer, Index 1 (100% over 30bp)
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGTG1347370.5109179964424542No Hit
AACTCCATCTAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1306250.4953254361110577RNA PCR Primer, Index 3 (100% over 34bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTG1107410.41992600283540393No Hit
AAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG1097560.41619091725018376RNA PCR Primer, Index 3 (100% over 38bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCC1063830.4034006190989677No Hit
AACACGGACCAAGGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAA1015150.3849413331813514No Hit
TCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC961140.3644609298861489RNA PCR Primer, Index 3 (100% over 42bp)
ACGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC844100.32007977081059813RNA PCR Primer, Index 3 (100% over 42bp)
ACTTCGGGATAAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA719660.27289255758980574RNA PCR Primer, Index 1 (100% over 28bp)
TCGAGGCCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG697750.26458436214085396RNA PCR Primer, Index 3 (100% over 37bp)
ATGTGTTGTTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG686480.2603108175169523RNA PCR Primer, Index 3 (100% over 37bp)
ATCCATTGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC656050.24877186783591154RNA PCR Primer, Index 3 (100% over 39bp)
ATGTGCTTGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG644050.24422150976254678RNA PCR Primer, Index 3 (100% over 37bp)
TGGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC613510.2326408484658335RNA PCR Primer, Index 3 (100% over 42bp)
AACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA605710.2296831157181464RNA PCR Primer, Index 3 (100% over 36bp)
TGTTGTTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA599230.22722592235852948RNA PCR Primer, Index 3 (100% over 40bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGTGGAATTCTCGGGTGCC597220.2264637373812409No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC567950.215364655647292No Hit
AGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT564500.21405642770119968RNA PCR Primer, Index 3 (100% over 43bp)
TGGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT534990.20286633880578353RNA PCR Primer, Index 3 (100% over 43bp)
TCCGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT520820.197493124314152RNA PCR Primer, Index 3 (100% over 41bp)
TGCCGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT518030.19643516606209474RNA PCR Primer, Index 3 (100% over 41bp)
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGTGGAATTCTCGGGT509910.19335609043245125No Hit
AGACGGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA497570.18867680554700783RNA PCR Primer, Index 3 (100% over 40bp)
TCGGTCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCAT492240.18665568816942166RNA PCR Primer, Index 3 (100% over 41bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCA474610.17997045376663665No Hit
AGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC459570.17426733831468616RNA PCR Primer, Index 3 (100% over 44bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA448330.17000516958596784RNA PCR Primer, Index 3 (100% over 40bp)
ACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGAAC442540.16780962181556935RNA PCR Primer, Index 1 (100% over 24bp)
AGAGGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC414230.1570745687274897RNA PCR Primer, Index 3 (100% over 42bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAGG404670.15344945012904246Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATT400210.15175823371177524No Hit
ACCAAGCGTTGGATTGTTCACCCACTAATAGGGTGGAATTCTCGGGTGCC388340.14725717118420528No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAG386810.14667700052985128No Hit
AGCCTCTGGCATGTTGGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGT363850.13797064874948006RNA PCR Primer, Index 1 (100% over 30bp)
ATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCTGGA357730.13564996613206406No Hit
TCACCAAGCGTTGGATTGTTCACCCACTAATAGGGTGGAATTCTCGGGTG350340.1328477039518836No Hit
AGTGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC349810.1326467298036433RNA PCR Primer, Index 3 (100% over 42bp)
ACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG334650.12689811077095922RNA PCR Primer, Index 3 (100% over 37bp)
TGGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC331650.12576052125261802RNA PCR Primer, Index 3 (100% over 42bp)
ATAAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA328590.12460017994391001RNA PCR Primer, Index 3 (100% over 36bp)
ATGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT328220.12445987723664793RNA PCR Primer, Index 3 (100% over 43bp)
TTCGAGGCCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA317470.12038351479592535RNA PCR Primer, Index 3 (100% over 36bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA312100.11834722955809462No Hit
AGACGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC306560.11624648091422458RNA PCR Primer, Index 3 (100% over 42bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC303030.1149079172476431No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGT299340.11350868214008344No Hit
AGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAGGAACTC299150.11343663480392185RNA PCR Primer, Index 1 (100% over 26bp)
ACCATCTGTGGGATTATGACTGATGGAATTCTCGGGTGCCAAGGAACTCC298630.11323945262074271RNA PCR Primer, Index 1 (100% over 27bp)
ACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTC290320.11008832965493762No Hit
ATCACTCGGCTCGTGCGTCGATGTGGAATTCTCGGGTGCCAAGGAACTCC283460.10748704162299744RNA PCR Primer, Index 1 (100% over 27bp)
TCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT278200.10549246800083924RNA PCR Primer, Index 3 (100% over 43bp)
AACGCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGT274620.10413494450895208No Hit
ACACTTCGAACGCACTTGCGGCCCCGGGTGGAATTCTCGGGTGCCAAGGA270050.10240201647601235RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGGGC267740.10152607254688964No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTC267170.10130993053840481No Hit
ATGGATGGCGCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG267060.1012682189227323RNA PCR Primer, Index 3 (100% over 37bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACATGGAATTCTCGGGTG266200.1009421099274745No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)