FastQCFastQC Report
Sun 2 Jul 2023
SRR944657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR944657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27324308
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1393080.5098317585938498RNA PCR Primer, Index 1 (100% over 32bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC1133290.41475524284091664RNA PCR Primer, Index 1 (100% over 24bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC1132150.4143380319091704No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1102150.40335879686321785No Hit
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC1043150.3817663012728447RNA PCR Primer, Index 6 (100% over 35bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC745410.27280105318678155No Hit
AACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATA632860.23161062303938307No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC620110.22694444814485326RNA PCR Primer, Index 6 (100% over 36bp)
CCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG605070.22144019164181578RNA PCR Primer, Index 6 (100% over 34bp)
ACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATAC571310.2090848924701039No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGG546100.1998586752864885Illumina Small RNA Adapter 2 (100% over 21bp)
ATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCG490910.17966054254695124No Hit
CGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA481620.17626063942772127RNA PCR Primer, Index 1 (100% over 22bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC435090.15923184587144898RNA PCR Primer, Index 6 (100% over 36bp)
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC428390.15677981671118624RNA PCR Primer, Index 1 (100% over 33bp)
TATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGG413460.15131581740331723No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC386310.14137960968673022RNA PCR Primer, Index 6 (100% over 35bp)
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC378410.1384884111246294No Hit
AGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG370400.13555695536736007No Hit
TAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG356750.1305614034214517No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA344350.126023319602458RNA PCR Primer, Index 1 (100% over 23bp)
TTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAG332300.12161332685900043No Hit
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC330570.1209801909713505RNA PCR Primer, Index 6 (100% over 36bp)
AATACAACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGA328030.12005061573745984No Hit
GGGGATGTAGCTCAGTGGTAGATGGAATTCTCGGGTGCCAAGGAACTCCA323740.11848058512588865RNA PCR Primer, Index 1 (100% over 28bp)
ATCCCGGACGAGCCCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC306720.11225169910981825RNA PCR Primer, Index 1 (100% over 33bp)
ATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAC300590.11000827541542864No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA296860.10864319052471522RNA PCR Primer, Index 6 (100% over 38bp)
TGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCA291680.10674744260678075No Hit
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC275640.10087721160221148RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG273760.10018917953933178No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)