FastQCFastQC Report
Tue 27 Jun 2023
SRR944659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR944659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36965794
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC28823017.797211119014513No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT20762815.61676289166141No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT15316404.143398083103531No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG14748673.9898155575935954No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCC8477782.2934121204051507No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC5894031.5944551333051307No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCA3941971.0663831541126914No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC3669940.9927934998501589No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC3320140.8981654769812331No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTG1944750.5260944753411761No Hit
ACTTCGGGATAAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA1944600.5260538972867728RNA PCR Primer, Index 1 (100% over 28bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTC1699600.4597764084277481No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAG1578740.42708131739304717No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCC1118700.3026311297411872No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGG1050950.2843033751689467No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGT1017740.2753193939240153No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAA909080.2459246513141311No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGATGGAATTCTCGGGTGCCAA874300.23651595309977652No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAG835000.2258845028460636No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGTGGAATTCTCGGGT740970.2004474731423326No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATT690430.18677537401198524No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAGG634330.17159918166508206Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTGGAATTCTCGGGTG627110.169646024646461No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAA626930.16959733098117682No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTGGAATTCTCGGGT606140.16397321264085388No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGGGC603260.16319411399630696No Hit
AACTTCGGGATAAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCC580530.1570451861523656RNA PCR Primer, Index 1 (100% over 27bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC575000.1555492085466905No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTC527550.1427130173370549No Hit
ACGAGAACTTTGAAGGCCGAAGTGGAGTGGAATTCTCGGGTGCCAAGGAA473810.12817525304609986RNA PCR Primer, Index 1 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGATGGAATTCTCGGGTGCCAAG428300.11586387134008268No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGGG419810.11356715346084545No Hit
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGC413590.1118845168049143No Hit
AAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAA403120.10905216860755107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)