FastQCFastQC Report
Sat 1 Jul 2023
SRR944660.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR944660.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57833548
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC3221460.5570227162960848No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC2749440.475405728177009No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC2078270.359353709372975RNA PCR Primer, Index 1 (100% over 24bp)
ACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATAC2004110.34653070221456933No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1956880.33836416192207336RNA PCR Primer, Index 1 (100% over 32bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1919050.3318229758271099RNA PCR Primer, Index 7 (100% over 35bp)
AACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATA1880410.325141732615125No Hit
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC1651130.285496922997012No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGG1619920.280100401241162Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1533170.26510045691818873No Hit
CGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGA1396100.24139968033778594RNA PCR Primer, Index 1 (100% over 22bp)
TATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGG1374540.23767174028472196No Hit
TAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGG1353880.23409941925057062No Hit
AGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG1340200.23173401016309772No Hit
TTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAG1244610.2152055412543598No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG1230490.2127640517576407RNA PCR Primer, Index 7 (100% over 36bp)
ATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCG1229480.21258941263641648No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA1198220.2071842453795157RNA PCR Primer, Index 1 (100% over 23bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG1160390.20064305928455228RNA PCR Primer, Index 7 (100% over 36bp)
TGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCA1100360.1902632707230758No Hit
ATACAACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAG938460.1622691383208929No Hit
AATACAACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGA934560.16159478923893794No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGG838550.1449936981213741Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC821870.14210955897085892RNA PCR Primer, Index 2 (100% over 34bp)
TAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCC736650.12737416698003726No Hit
ATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAAC734340.12697474483149468No Hit
TCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC718950.12431365960808768RNA PCR Primer, Index 1 (100% over 33bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA703760.1216871563888835RNA PCR Primer, Index 7 (100% over 37bp)
GGGGATGTAGCTCAGTGGTAGATGGAATTCTCGGGTGCCAAGGAACTCCA702940.12154537017165193RNA PCR Primer, Index 1 (100% over 28bp)
TTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATA682180.1179557581353992No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA677560.11715691383831406RNA PCR Primer, Index 7 (100% over 35bp)
CATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTG666410.11522896710400683No Hit
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG663890.1147932338510513RNA PCR Primer, Index 7 (100% over 36bp)
GTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCA662670.11458228362541409No Hit
AGTGGTTAGGATTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCG648740.11217364703268767No Hit
TCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCT627540.10850795458718873No Hit
TACAACGATGGTTTTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGA611350.10570854134697044No Hit
CTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTG602220.10412987285511172No Hit
TTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTC588870.10182152407457346No Hit
TTCGGCGCTCTCACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAACC588520.10176100556721854No Hit
TTTCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATT581390.100528157117388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)