FastQCFastQC Report
Thu 29 Jun 2023
SRR944662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR944662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39628591
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC404272610.201538581071429No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT21084215.320454113546455No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCC12249603.0911015736088117No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG10588142.671843669637409No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT10010372.5260474186427673No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC9807892.4749529954269636No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCA5600881.4133432097043268No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC5504901.3891233226031177No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC2397530.6050000616978787No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTG2053460.5181763843180799No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCC1683190.4247413187110286No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAG1580660.3988685845530062No Hit
ACTTCGGGATAAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA1556040.3926558983638858RNA PCR Primer, Index 1 (100% over 28bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTC1548290.3907002396325421No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAA1444540.36451964693874683No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAG1293250.3263426650722959No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTC1284560.3241498038625698No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGATGGAATTCTCGGGTGCCAA1198850.30252148000921864No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGTGGAATTCTCGGGT1073560.2709054177575983No Hit
ACGAGAACTTTGAAGGCCGAAGTGGAGTGGAATTCTCGGGTGCCAAGGAA947640.23913037937684942RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAA846180.21352765229528348No Hit
AAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAA826810.20863976718223468No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTGGAATTCTCGGGTG818850.20663111640784806No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTGGAATTCTCGGGT794980.20060768751530936No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATT784340.19792275733447096No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGTGGAATTCTCGGGTGCCAAGG746820.18845484564414616Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGG679980.1715882353727893No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGATGGAATTCTCGGGTGCCAAG678640.17125009566956342No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGT649080.16379083475362524No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC647500.16339213271549322No Hit
AACGAGAACTTTGAAGGCCGAAGTGGAGTGGAATTCTCGGGTGCCAAGGA632780.15967764284125066RNA PCR Primer, Index 1 (100% over 22bp)
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGC617470.15581427056036384No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGC608370.15351794869517313No Hit
AGCAGTTGAACATGGGTCAGTCGGTCCTGTGGAATTCTCGGGTGCCAAGG562500.14194297243623927Illumina Small RNA Adapter 2 (100% over 21bp)
AACTTCGGGATAAGGATTGGCTCTGGAATTCTCGGGTGCCAAGGAACTCC547650.13819567796392257RNA PCR Primer, Index 1 (100% over 27bp)
AAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAAT533550.1346376407881875No Hit
ATTAATGTGAATTGCAGGACACATTGATCATCGACACTTCGTGGAATTCT516090.13023173092376664No Hit
ACGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT489910.12362538955775643RNA PCR Primer, Index 1 (100% over 30bp)
AGAGGGCGTGAAACCGTTAAGAGGTGGAATTCTCGGGTGCCAAGGAACTC460230.11613584747436516RNA PCR Primer, Index 1 (100% over 26bp)
ATTAATGTGAATTGCAGGACACATTGATCATCGACACTTGGAATTCTCGG451410.11391018166656494No Hit
AACGAGAACTTTGAAGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG439050.11079122141889929RNA PCR Primer, Index 1 (100% over 29bp)
ACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCG437530.11040765996449381No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCAAGCCGGATCCGTGGAATTCT435220.10982474749102232No Hit
ATTAATGTGAATTGCAGGACACATTGATCATCGACACTGGAATTCTCGGG426260.10756375365452685No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATT415460.10483844858375105No Hit
TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGGAATT398960.10067478805895472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)