FastQCFastQC Report
Tue 27 Jun 2023
SRR948549.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR948549.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26819693
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA898896933.516300876374686Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3046591.135952600203142Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2910801.0853218938785019Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC908660.338803281603559Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCAGTCAC577100.21517770542712772Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA407200.15182873271517314Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACTATCT393800.14683240408456577TruSeq Adapter, Index 15 (97% over 36bp)
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA338260.12612374049173494Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCATCAC335470.125083460127601Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC327550.12213040619070471Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCGTCAC325730.12145180036177147Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGACTCCAGTCAC325610.12140705712030335Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA324640.12104538258510267Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)