FastQCFastQC Report
Tue 27 Jun 2023
SRR948550.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR948550.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15222810
Total Bases761.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6777574.452246332970063Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA230410.1513583891541706Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA218930.14381707450858283Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)