FastQCFastQC Report
Tue 27 Jun 2023
SRR948553.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR948553.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41214129
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA34256788.311901969346483No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG7106741.7243455514976431No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA6100161.480113773604193No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA5835571.4159149159745679No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4742791.1507679805631705No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA4516741.0959202850071148No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG4471871.0850332418768331No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3959360.960680256035497No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA2796530.678536722200292No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2270120.5508111065503773No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2059000.49958595509806847No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1990570.482982425759865No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1840670.44661140357958307No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1511270.36668735617341325No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG1434570.34807723341672464No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1304880.31660986939697305No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1278900.3103062059130256No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA1278700.31025767886542016No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG1257860.3052011605049326No Hit
ACACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1228340.2980385682783688No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1226900.2976891735356096No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1164320.2825050603398655No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1148690.27871267156949986No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1050340.2548494959095217No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1022690.2481406315780688No Hit
GCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG981090.23804700567613596No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG970850.23556242083873713No Hit
CTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA885420.21483409245407079No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG810640.19668982935439447No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA789910.19166000087008997No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA785210.190519615251362No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG774460.18791128644256924No Hit
CACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGA772880.18752792276648622No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG754760.1831313722534328No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA668140.16211430793551404No Hit
CTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA656160.15920753778394783No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA638290.15487164608040122No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA637920.15478187104233113No Hit
TCATGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA635140.15410734508061544No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA628420.15247683628107245No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG622560.15105499378623288No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT612720.1486674630440449No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG584920.14192220342688788No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA579850.14069204277008984No Hit
ATTAAATCAGTTATCGTTTATTTGATACTGTAGGCACCATCAATAGATCG564450.1369554601044705No Hit
TCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG550390.1335440086578076No Hit
GCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGATC533380.12941678325896444No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA523600.1270438106310581No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG514790.1249061941840382No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG500810.12151415355641751No Hit
CCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA499700.12124482844220728No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA478560.11611551951031163No Hit
GCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA446920.10843854057913004No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGA436560.10592483951316792No Hit
TGCGGAGAATTTGGACAAACTTGGTCATTCTGTAGGCACCATCAATAGAT428630.10400074207561197No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA427790.1037969284756691No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)