FastQCFastQC Report
Tue 27 Jun 2023
SRR948554.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR948554.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76161853
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC32823604.309716571628056No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA28365953.724430129083125No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA19584582.5714421627845634No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT16301502.1403759701067147No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG15804592.0751320218009925No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG13703401.7992471900598321No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA12835021.6852294809581378No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAA9698861.2734537853221612No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG8378951.100150491348996No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA7993471.0495372269894747No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAAG7951491.0440252812651498No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC7024260.922280606801938No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG6817200.8950937682674291No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG5703880.7489156021453417No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA5582080.7329233441838658No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG5553880.729220703178007No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA5452010.7158452407926577No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATAGATCGGA5023890.6596333731533554No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA4377800.5748021913279867No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG4377320.5747391676512913No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG4347240.5707896839117084No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4077460.5353677516223246No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA3826670.5024391935422055No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG3160890.415022727979058No Hit
GCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA2831610.37178848576596474No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA2717510.35680723261814545No Hit
GGAATCCGGTTAAGATTCCGGAACCTGCTGTAGGCACCATCAATAGATCG2513830.3300641858070339No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2450070.3216925407526521No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT2371170.31133302389583406No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA2369270.3110835551755811No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA2003270.26302800169528434No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1937080.2543372992776318No Hit
TTCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1871180.2456846736646494No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG1861790.24445177298929427No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1834620.2408843702896777No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG1827390.23993507615945214No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATAGATC1743850.22896633042791117No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATAGAT1556050.2043083169208081No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAA1538270.20197381489654667No Hit
TCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAAG1526820.2004704376087068No Hit
CCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG1473570.193478748475303No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGG1467090.19262792883991414No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG1463720.19218545010978133No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC1391330.1826806918681456No Hit
CTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAA1381370.18137295057671457No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAA1357970.17830054633781034No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA1322390.17362891630275853No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG1263700.16592295883347272No Hit
TTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAAG1256930.16503406239341367No Hit
TTGTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1240080.16282166874274973No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1226580.16104912783568961No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1212110.15914922658197406No Hit
GAATCCGGTTAAGATTCCGGAACCTGCTGTAGGCACCATCAATAGATCGG1150090.15100604235561338No Hit
TCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAGA1147550.15067254206643316No Hit
TTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCG1139690.14964052936054484No Hit
TGTGCCCGAGTTGTAATTTGGAGAGGGCCTGTAGGCACCATCAATAGATC1121010.14718785794247943No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1055130.138537858316026No Hit
CCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATAGATCGGAA1050250.1378971176029554No Hit
TTCGGTGCCCGAGTTGTAATTTGGAGAGGGCCTGTAGGCACCATCAATAG1045320.1372498119235623No Hit
ACACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1038370.13633728160474248No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGA987630.12967515378072536No Hit
GCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG964680.12666183423872315No Hit
TCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGAAG954220.12528844328406769No Hit
CCAACGGGGCCTTTCCTTCTGGCTGTAGGCACCATCAATAGATCGGAAGA936250.12292899438778099No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA916290.12030825983186098No Hit
CTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA890720.11695093605456264No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGAT842560.1106275604927837No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGAT829570.10892198224221251No Hit
GCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA829350.10889309639039377No Hit
GTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAA823760.10815913315554442No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG791680.10394705076306376No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)