FastQCFastQC Report
Wed 28 Jun 2023
SRR948563.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR948563.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97125729
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG13886341.42972826489673No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA12645581.3019804463964435No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC11946981.230053058340494No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG7546520.7769846443057328No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG7061760.7270740794130873No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG6930260.7135349274958852No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG6273470.6459122690342947No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT6127340.6308668221167225No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA5177370.5330585472362324No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4856600.5000322828979744Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA4505310.4638636998029636No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4381870.4511544000869224No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG3807770.392045448636993No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG3279240.3376283538628575No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC3239970.33358514096712727No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA3043530.31335981014876085No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2776690.2858861424865084No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA2602660.26796813025722566No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT2577090.26533546018480847No Hit
TTGCCTTGTTGTAGACGGCCTTGGTCTGTAGGCACCATCAATAGATCGGA2447190.25196104319587653No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG2358990.24288003027498511No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT2231220.2297249166593128No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG2201410.22665569902697974No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA2063980.21250599828187647No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG2029200.2089250727786043No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1965330.2023490603607207No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1924350.19812978701040174No Hit
TTGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGA1846860.19015146851561854No Hit
CCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA1814020.18677028411287394No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1812820.18664673291667133No Hit
CTTTGAACGCACATTGCGCCCCTTGGCTGTAGGCACCATCAATAGATCGG1803320.1856686192800674No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1742810.17943855021155106No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG1645170.1693856012138658No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1625420.16735215444303125No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1616720.16645640827056238No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC1562740.16089866362804855No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTCTGTAGGCACCATCAATAGA1500460.15448635654513337No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCCTGTAGGCACCATCAATAGAT1488240.15322819353047018No Hit
TCTTTGAACGCACATTGCGCCCCTTGGCTGTAGGCACCATCAATAGATCG1483530.15274325508537495No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCTGTAGGCACCATCAATAG1405250.1446835987197584No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1394500.14357678592044337No Hit
CCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1363950.14043137838378542No Hit
GGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG1342960.13827026204354154No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1326990.1366260015407452No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG1317560.13565509505725307No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG1241030.12777561751943195No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1240940.12776635117971677No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1233290.12697871230392516No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1219650.12557434704042222No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCG1201620.12371799031747807No Hit
GATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGG1182200.12171852012559925No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG1170090.12047168263725466No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGG1147230.11811803234959503No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC1144910.11787916670360332No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA1123040.11562744615281086No Hit
CTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCACCATCAATAGATCG1120080.1153226865355111No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1086890.11190546636720738No Hit
TATCTGGTTGATCCTGCCAGTAGTCCTGTAGGCACCATCAATAGATCGGA1040080.10708594012200413No Hit
TCTTTGAACGCACATTGCGCCCCTTGGTCTGTAGGCACCATCAATAGATC1039460.10702210533729944No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1027030.1057423208633008No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1014540.10445635882949203No Hit
GGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATCG1011390.1041320369394602No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC1000810.1030427272262739No Hit
GGAATCCGGTTAAGATTCCGGAACCTGCTGTAGGCACCATCAATAGATCG985050.10142008818281303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)