FastQCFastQC Report
Tue 8 Aug 2023
SRR950388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR950388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182247
Total Bases56.7 Mbp
Sequences flagged as poor quality0
Sequence length26
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNN1931358.850281384279599No Hit
GTGGGGGGGCCGGGCCACCCCTCCCN805013.688904143298169No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC544882.49687592651061No Hit
CGTGGGGGGCCCAAGTCCTTCTGATC409531.8766436613270634No Hit
CGGGGGGGATGCGTGCATTTATCNNN376601.7257441527013213No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCC333011.5259959115535502No Hit
AGCGCCGCGGAGCCTCGGTTGGCCTC319341.463354056621455No Hit
TCTCGCTTCTGGCGCCAAGCGCCCGG223381.0236238152693073No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCG222181.0181248960360583No Hit
TCGTGGGGGGCCCAAGTCCTTCTGAT218881.0030028681446235No Hit
CGTGGGGGGGCCGGGCCACCCCTCCC184850.8470626835550696No Hit
CACTCGCCGAATCCCGGGGCCGAGGG183120.8391350749938022No Hit
CCGCCGAGGGCGCACCACCGGCCCGT173490.7950062481469788No Hit
GTGGGGGGCCCAAGTCCTTCTGATCG173140.7934023967039479No Hit
CGCGACCTCAGATCAGACGTGGCGAC142110.6512095101975166No Hit
CTTCCCGTGGATCGCCCCAGCTGCGG133470.6116172917181236No Hit
GGGGGGGGATGCGTGCATTTATCNNN103320.4734569459827417No Hit
GGCTGGGCGCGCGCCGCGGCTGGACG99640.4565935936674447No Hit
CCGCGAGGGGGGTCTCCCCCGCGGGG83370.3820374137299765No Hit
TTCCCGTGGATCGCCCCAGCTGCGGC82550.37827981892058965No Hit
CGCGAGGGGGGTCTCCCCCGCGGGGG78340.35898777727727427No Hit
CCCTTCCCGTGGATCGCCCCAGCTGC77920.3570631555456371No Hit
AGCCTCGGTTGGCCTCGGATAGCCGG76230.3493188442921447No Hit
CTCTCGCTTCTGGCGCCAAGCGCCCG75730.3470276279449576No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGN74520.3414828843847649No Hit
ATCTTGAGTTGTGATGCATAGTGANN73130.33511330293958475No Hit
CTTTGGTGACTCTAGATAACCTCGGG71120.32590261322389263No Hit
GATGCGTGCATTTATCAGATCAAAAC70990.32530689697362397No Hit
CGGCGGCGGCGGCGACTCTGGACGCG68820.315363018026832No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCG66340.30399858494478393No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTG65500.3001493414815096No Hit
TCCCGTGGATCGCCCCAGCTGCGGCG65420.2997827468659597No Hit
ATGCGTGCATTTATCAGATCAAAACC59830.274166948104408No Hit
GGGGGGGATGCGTGCATTTATCNNNN56640.2595489878093543No Hit
CCCTCGCCCGTCACGCACCGCACGTT55350.2536376496336116No Hit
TCCGCCGAGGGCGCACCACCGGCCCG54320.24891774395840618No Hit
CAGCGCCGCGGAGCCTCGGTTGGCCT54210.248413676362025No Hit
CTCCCGGGGCTACGCCTGTCTGAGCG52800.24195244626295742No Hit
GCCTCGGTTGGCCTCGGATAGCCGGT52720.24158585164740748No Hit
GGATGCGTGCATTTATCAGATCAAAN52590.24099013539713882No Hit
CGGGACCGGGGTCCGGTGCGGAGTGC52440.2403027704929827No Hit
ATTGATCATCGACACTTCGAACGCAC51740.23709506760692078No Hit
CTGGGCGCGCGCCGCGGCTGGACGNN51400.23553704049083354No Hit
TCGTGGGGGGGCCGGGCCACCCCTCC50110.22962570231509083No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCG47050.2156034582703058No Hit
GGCTCTCGCTTCTGGCGCCAAGCGCC46210.21175421480703147No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGC42830.1962655923000467No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGC40830.1871007269112983No Hit
CCTCCCGGGGCTACGCCTGTCTGAGC40780.1868716052765796No Hit
CCAACTTCTTAGAGGGACAAGTGGCG40230.1843512672946738No Hit
CGGGGGGCCGGCGGCGGCGGCGACTC38650.17711102363756256No Hit
CCGAGGGGCTCTCGCTTCTGGCGCCN38640.17706519931061881No Hit
GAGCCTCGGTTGGCCTCGGATAGCCG37970.1739949694053881No Hit
TGGGCGCGCGCCGCGGCTGGACGAGG36050.16519669863218966No Hit
GGCTTTGGTGACTCTAGATAACCTCG35930.16464680670886478No Hit
CGCCCCGGGCGGGTCGCGCCGTCGGG35760.16386779315082114No Hit
CCCGTGGATCGCCCCAGCTGCGGCGG35630.1632720769005525No Hit
GCTCGTGGGGGGCCCAAGTCCTTCTG34840.1596519550719969No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGG32760.15012049506769856No Hit
GGGCTGGGCGCGCGCCGCGGCTGGAC32700.14984554910603612No Hit
TGCGTGCATTTATCAGATCAAAACCN31710.14530894073860567No Hit
CTCGACTGCATAATTTGTGGTAGTGG30880.1415055216022751No Hit
AGCACTCGCCGAATCCCGGGGCCGNN30690.14063485939034398No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGT30070.137793751119832No Hit
TGGGGGGGCCGGGCCACCCCTCCCAC29840.13673979160012592No Hit
GTCCGCCGAGGGCGCACCACCGGCCC29810.1366023186192947No Hit
TTAAATGGATTTTTGGAGCAGGGNNN29620.1357316564073636No Hit
GGGGATGCGTGCATTTATCAGATCAN29380.1346318725607138No Hit
GGCGGCGGCGGCGACTCTGGACGCGN28630.13119504803993315No Hit
GTTGAACCCCATTCGTGATGGGGATC27770.12725415592277134No Hit
CCTTCCCGTGGATCGCCCCAGCTGCG27730.12707085861499637No Hit
CGGCTTTGGTGACTCTAGATAACCTC27610.12652096669167148No Hit
CCGTGGATCGCCCCAGCTGCGGCGGG27150.12441304765225936No Hit
GGGATGCGTGCATTTATCAGATCAAN26580.12180106101646607No Hit
GGGGGGGGGATGCGTGCATTTATCNN26460.12125116909314115No Hit
GGGGGATGCGTGCATTTATCAGATCN26370.12083875015064748No Hit
GCCGATTTAGCTCAGCGGTAGAGCNN26090.1195556689962227No Hit
TCGCTTCTGGCGCCAAGCGCCCGGNN25850.1184558851495729No Hit
TCGACTGCATAATTTGTGGTAGTGGG25790.11818093918791045No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGN25220.11556895255211716No Hit
CCCGCGAGGGGGGTCTCCCCCGCGGG24590.11268201995466143No Hit
GTGGGGGGGCCGGGCCCCCCCTCCCN24380.11171970908884284No Hit
ATTAAATGGATTTTTGGAGCAGGGNN24170.11075739822302426No Hit
CATTGATCATCGACACTTCGAACGCN23110.1059000195669876No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT22950.10516683033588772No Hit
GGAGCCTCGGTTGGCCTCGGATAGCC22910.10498353302811275No Hit
CGACTGCATAATTTGTGGTAGTGGGG22860.10475441139339405No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCC22500.10310473562341935No Hit
TTGATCATCGACACTTCGAACGCACT22360.10246319504620695No Hit
CTCGCCGGCCGAGGTGGGATCCCGNN22310.10223407341148824No Hit
CGGGCGGGTCGCGCCGTCGGGCCCGG22290.10214242475760076No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCC21950.10058439764151353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)