FastQCFastQC Report
Tue 27 Jun 2023
SRR950450.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR950450.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172154977
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA2085802212.11584025247205No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA71415314.148315154432044No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA54175393.1468965314897637No Hit
TCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA42419832.4640490062625373No Hit
TGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA22067471.2818374690381447No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA19366611.1249520831454092No Hit
CACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAA12292590.7140420924339584No Hit
TCACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAA11033490.6409045031558978No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAA10494620.6096030555073642No Hit
TCAATATAGATGGATACGAATAAGGCGTCAAAAAAA10374810.6026436284790071No Hit
TAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA10069290.5848968281643115No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAA9564940.5556005505434792No Hit
GAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAA9551610.5548262482123884No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAA9111520.5292626538470625No Hit
AAATATAGATGGATACGAATAAGGCGTCAAAAAAAA8994970.5224925910797223No Hit
AGGGAGGTAGTGACAATAAATAACGAAAAAAAAAAA8260050.4798031485316861No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAA7917350.45989666624625086No Hit
TGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA7504020.43588748526277No Hit
TCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAA7089430.41180511441153395No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAA6715210.39006772368829046No Hit
CCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA6086620.3535546927580258No Hit
TCAGGGAGGTAGTGACAATAAATAACGAAAAAAAAA5734950.3331271683188108No Hit
GCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA5129090.2979344593679682No Hit
TTAAGCCATGCATGTCTAAGTATAAGCAAAAAAAAA5077180.29491915299085425No Hit
AAGCCATGCATGTCTAAGTATAAGCAAAAAAAAAAA4409890.2561581475509709No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAA4372400.25398045854927565No Hit
TTTAAGCCATGCATGTCTAAGTATAAGCAAAAAAAA4291620.24928817480542545No Hit
ACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAA4217160.24496300214428304No Hit
TATAGATGGATACGAATAAGGCGTCAAAAAAAAAAA3944980.22915282896526423No Hit
CAATATAGATGGATACGAATAAGGCGTCAAAAAAAA3875600.2251227392630072No Hit
TTCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAA3779360.21953242745924215No Hit
AGCCATGCATGTCTAAGTATAAGCAAAAAAAAAAAA3715650.21583169216188272No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAA3612720.20985277701265648No Hit
ACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAA3576710.20776105706197504No Hit
CACGTTCTAGCATTCAAGGTCCCGAAAAAAAAAAAA3550440.2062351064064793No Hit
TAAGCCATGCATGTCTAAGTATAAGCAAAAAAAAAA3448640.20032182978944607No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAA3416740.19846884821691796No Hit
TAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA3372840.19591882028481816No Hit
ATGAGGTAAAGCGAATGATTAGAGGTAAAAAAAAAA3296440.19148095846221164No Hit
TTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAA3290370.19112836917866163No Hit
AAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAA3075200.1786297470795747No Hit
AGAGGTGCACAATCGACCGATCCGAAAAAAAAAAAA2976870.17291803303485093No Hit
ATGGGGTCTTATGGCTGGAAGAGGCCAGCAAAAAAA2751120.1598048483954083No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAA2674940.1553797657560606No Hit
AGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAA2667450.1549446926532946No Hit
AAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAA2631020.15282857608002817No Hit
TTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAA2581100.1499288632242099No Hit
AGATGGGGTCTTATGGCTGGAAGAGGCCAGCAAAAA2479510.14402778491846913No Hit
TTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAA2415660.14031891741358135No Hit
TCACGTTCTAGCATTCAAGGTCAAAAAAAAAAAAAA2408270.13988965303047846No Hit
CACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAA2351970.13661934386015456No Hit
AAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAA2326410.13513463511426682No Hit
ACACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAA2135250.12403068660628963No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAAAAAAAA2055800.11941565883395865No Hit
TAATATAGATGGATACGAATAAGGCGTCAAAAAAAA2051090.11914206813782677No Hit
AAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAA2027990.11780025389565126No Hit
TTCAGGGAGGTAGTGACAATAAATAACGAAAAAAAA1990760.11563766756508005No Hit
GAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAA1905480.11068399143639047No Hit
TTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAA1898080.11025414618132127No Hit
GGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA1882530.1093508902737096No Hit
TAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAA1871710.10872238680616246No Hit
TATGAGGTAAAGCGAATGATTAGAGGTAAAAAAAAA1868020.10850804505059415No Hit
ATTAAGCCTTTGTTGTCTGATTTGTAAAAAAAAAAA1864570.10830764422221728No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA1820320.10573728577129664No Hit
GATGGGGTCTTATGGCTGGAAGAGGCCAGCAAAAAA1759150.1021840919533799No Hit
TTGGGGTCTTATGGCTGGAAGAGGCCAGCAAAAAAA1748520.10156662505319262No Hit
TTTCGGTGCCCGAGTTGTAATTTGAAAAAAAAAAAA1742270.10120358007424902No Hit
TGGGGTCTTATGGCTGGAAGAGGCCAGCAAAAAAAA1738670.10099446616637753No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAA1735850.10083066027187816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)