FastQCFastQC Report
Thu 29 Jun 2023
SRR950452.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR950452.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105279209
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length36
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA103218949.804304285758834No Hit
AGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAA40839303.8791419870945267No Hit
AAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA20895981.9848154444245492No Hit
AGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAA17559231.6678725236242988No Hit
AAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAA9468400.8993608605095047No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAAAA8992640.8541705513763881No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAA6795600.6454835731146118No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAA6152070.5843575439477323No Hit
AGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAA6138220.5830419945499401No Hit
ATGTACACGGAGTCGACCCGCAACGCGAAAAAAAAA5976590.5676894855849458No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT5645440.5362350319330381No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAA5481670.5206792539636198No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAA5421800.5149924711155457No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTAAA4754870.45164378087225177No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCAAA4717690.44811221938417106No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT4200120.3989505658234951No Hit
AGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAA4081880.3877194784014762No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAAA3790620.3600539969862425No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAAAA3773130.3583927003098969No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT3731560.35444415240619825No Hit
ATGTACACGGAGTCGACCCGCAACGCAAAAAAAAAA3438980.32665329010973093No Hit
AAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAA3377310.320795533332702No Hit
AGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAA3033850.28817180797777464No Hit
TCGGGATAAGGATTGGCTCTAAGGGTCGGAAAAAAA2832520.26904837402416276No Hit
TTGCCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCA2820780.26793324406531205No Hit
ATAAAAAAAAAAAAAAAAAAAATCGTATGCCGTTTT2565790.24371288731852078No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAAAAAAAA2376890.22577012332985902No Hit
ATAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC2376890.22577012332985902No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT2369260.22504538384212214No Hit
GCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT2319440.2203132054307133No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAA2305060.21894731370939538No Hit
ATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAA2219850.21085359788369992No Hit
TCAGGGAGGTAGTGACAATAAATAACGAAAAAAAAA2215290.21042046393034736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2194840.2084780101263869No Hit
AAATATAGATGGATACGAATAAGGCGTCAAAAAAAA2145700.20381042186591655No Hit
GCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG1973790.18748146179555736No Hit
ATGAGGTAAAGCGAATGATTAGAGGTAAAAAAAAAA1949410.18516571491337858No Hit
ACAAAAAAAAAAAAAAAAAAAATCGTATGCCGTTTT1943920.18464424443006597No Hit
AAGCGTGTTACCTATACTCTACCGTCAGGGTTGAAA1851040.1758219896959902No Hit
ATACTGCCAGCTGGGACTGAGGACTGCGACGAAAAA1841920.1749557217892851No Hit
GTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT1839710.17474580379873483No Hit
ACAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG1798080.17079155676407104No Hit
AGAAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTT1750630.16628449402578624No Hit
AGATGGGGTCTTATGGCTGGAAGAGGCCAGCAAAAA1746560.16589790297531587No Hit
ACAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC1718540.1632364088145837No Hit
AAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAA1717820.16316801924300173No Hit
ATATAGATGGATACGAATAAGGCGTCAAAAAAAAAA1691600.16067749901122452No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAA1648690.15660167051597054No Hit
AAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAA1643060.15606690206040585No Hit
ATAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCT1571450.1492649892534812No Hit
ATAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG1567820.14892019183008867No Hit
TCAAAAAAAAAAAAAAAAAAAATCGTATGCCGTTTT1551960.14741372154496335No Hit
TCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC1550460.1472712432708342No Hit
AGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAA1527440.14508467669053252No Hit
AGGGAGGTAGTGACAATAAATAACGAAAAAAAAAAA1511750.1435943539431418No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGAAAAAA1419910.1348708841457956No Hit
ACAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCT1391970.1322169888263503No Hit
AGAGGTGCACAATCGACCGATCCGAAAAAAAAAAAA1349220.12815635801367012No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAAAAAA1348720.12810886525562706No Hit
AGATTAAGCCATGCATGTCTAAGTATAAGCAAAAAA1343680.1276301382545532No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAA1259840.11966655258589566No Hit
TAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAA1246890.11843649015258084No Hit
GTAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG1224980.11635535749513469No Hit
TCAATATAGATGGATACGAATAAGGCGTCAAAAAAA1220710.11594976934144709No Hit
TCAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG1203660.11433026629217931No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTAAAAAA1198060.11379834740209721No Hit
TTAAAAAAAAAAAAAAAAAAAATCGTATGCCGTTTT1187510.112796250207389No Hit
CCCGCGCAGGTTCGAGTCCTGCAGTTGTCGCCAAAA1183510.11241630814304464No Hit
AATATAGATGGATACGAATAAGGCGTCAAAAAAAAA1177900.11188343939780171No Hit
AGAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCTG1163840.11054794304163132No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTGAAA1163290.11049570100778397No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACAAAAA1155180.10972536847232582No Hit
AATTCAGGGAGGTAGTGACAATAAATAACGAAAAAA1149950.10922859422319559No Hit
TCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCT1120860.10646546556025133No Hit
TTACTGCCAGCTGGGACTGAGGACTGCGACGAAAAA1085600.10311627626305589No Hit
GCAAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTC1081580.10273443448838983No Hit
GCAAAAAAAAAAAAAAAAAATCGTATGCCGTCTTCT1062420.1009145120001804No Hit
ATTCAGGGAGGTAGTGACAATAAATAACGAAAAAAA1058790.1005697145767879No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)