FastQCFastQC Report
Fri 14 Jul 2023
SRR9596293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9596293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53007899
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGATCGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT1285750.24255818929929668TruSeq Adapter, Index 12 (97% over 41bp)
CCGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT1087150.2050920750509278TruSeq Adapter, Index 12 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT792300.1494682896222693TruSeq Adapter, Index 12 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT725400.1368475290824109TruSeq Adapter, Index 12 (100% over 48bp)
GGCCAGATCGGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT649350.12250061071086783TruSeq Adapter, Index 12 (100% over 40bp)
GCGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT530400.10006055889896712TruSeq Adapter, Index 12 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)