FastQCFastQC Report
Fri 14 Jul 2023
SRR9596294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9596294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50803609
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT2423870.4771058686007917TruSeq Adapter, Index 12 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT1951970.38421876682028633TruSeq Adapter, Index 12 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT1481220.2915580269110409TruSeq Adapter, Index 12 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT1136340.22367308590222398TruSeq Adapter, Index 12 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT1118370.2201359356182747TruSeq Adapter, Index 12 (100% over 48bp)
ATGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT982130.19331894314831058TruSeq Adapter, Index 12 (98% over 50bp)
CAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT882770.17376127747144893TruSeq Adapter, Index 12 (98% over 50bp)
TTGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTT692590.13632692905734314TruSeq Adapter, Index 12 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)