FastQCFastQC Report
Fri 14 Jul 2023
SRR9596296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9596296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48842936
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT1265520.2590999034128497TruSeq Adapter, Index 1 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT919870.18833224931441467TruSeq Adapter, Index 1 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT857910.17564668921622564TruSeq Adapter, Index 1 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT569050.11650610028848389TruSeq Adapter, Index 1 (100% over 48bp)
GCGCAGATCGGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT562800.11522648843222694TruSeq Adapter, Index 1 (97% over 41bp)
CTGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTT543030.1111788202085149TruSeq Adapter, Index 1 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)