FastQCFastQC Report
Fri 14 Jul 2023
SRR9596303_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9596303_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60336502
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT914370.151545079626923TruSeq Adapter, Index 4 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT640420.10614138685069943TruSeq Adapter, Index 4 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT604040.10011186926282202TruSeq Adapter, Index 4 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)