FastQCFastQC Report
Fri 14 Jul 2023
SRR9596304_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9596304_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67408221
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT1631280.24200015603438046TruSeq Adapter, Index 4 (100% over 48bp)
ACGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT1298220.19259075239502316TruSeq Adapter, Index 4 (98% over 50bp)
TCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT1014540.15050686473390243TruSeq Adapter, Index 4 (100% over 48bp)
CTGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT745310.11056663251801291TruSeq Adapter, Index 4 (100% over 48bp)
GCGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTT690340.10241184083466615TruSeq Adapter, Index 4 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)